PLASMA GLYCEROL SPECIFIC ACTIVITY BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

通过高效液相色谱法测定血浆甘油的比活性

• 批准号: 6336786
• 负责人: RL L JUDD
• 金额: $ 0.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6336786
• 关键词: biological products; biomedical resource; proteins; spectrometry

Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determinatin of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described which permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H] glycerol and [2H5] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66(0.14 vs 1.70(0.10 (mol.kg-1.min-1, respectively, P=NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radiotracers.

测量血浆甘油比活度的先前方法 (SA)是次优，使甘油动力学测定 体内放射性示踪剂很难。 一种新型高性能液体 色谱法（HPLC）的方法，它允许准确的 和甘油SA的特定测量。 该方法涉及 从血浆中分离甘油并形成三苯甲酰基 衍生物 测定了5例正常人的甘油出现率， 使用[2- 3 H]甘油和[2 H5]甘油的志愿者。 有 甘油出现率之间的密切协议，测量使用 两种方法（1.66（0.14）对1.70（0.10（mol·kg-1·min-1， P=NS）。 该HPLC方法提供了改进的专属性 现有的甘油周转率测量方法， 放射性示踪剂