PLASMA GLYCEROL SPECIFIC ACTIVITY BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

通过高效液相色谱法测定血浆甘油的比活性

• 批准号: 6486716
• 负责人: RL L JUDD
• 金额: $ 15.75万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6486716
• 关键词: biological products; biomedical resource; proteins; spectrometry

Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determinatin of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described which permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H] glycerol and [2H5] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66(0.14 vs 1.70(0.10 (mol.kg-1.min-1, respectively, P=NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radiotracers.

以往测定血浆甘油比活度的方法 (Sa)是次优的，使得甘油动力学的测定在 用放射性示踪剂进行活体检查很困难。一种新型高性能液体 介绍了一种高效液相色谱(HPL C)方法，该方法能够准确地测定 甘油SA的特异性测定。该方法涉及 血浆中甘油的分离及三苯甲酰基的形成 衍生品。甘油出现率在五个人中被测量 受试者同时使用[2-3H]甘油和[2H5]甘油。曾经有过 甘油出现率与使用 两种方法(1.66(0.14比1.70(0.10(摩尔·kg-1.min-1， P=NS)。这种高效液相色谱方法具有更好的特异性。 现有的测量甘油周转率的方法 放射性示踪剂。