CHARACTERIZATION OF EXTRACELLULAR DOMAIN OF CA++ SENSING RECEPTOR

CA 传感受体胞外域的表征

• 批准号: 6279483
• 负责人: ALLEN M. SPIEGEL
• 金额: $ 2.52万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6279483
• 关键词: biological products; biomedical resource; proteins; spectrometry

We are currently working on a G protein-coupled receptor activated by extracellular Ca++ (in the millimolar range) with the standard seven transmembrane domain structure but with a unique, approximately 600 amino acid extracellular amino terminal domain (ECD). The latter contains multiple asp and glu residues speculated to be involved in Ca++ binding, 9 putative glycosylation sites, multiple cysteines conserved in the related ECD of the metabotropic glutamate receptor family, and a putative signal peptide. Mass spectrometry is being used to characterize both the intact protein and digested fragments in an effort to help define number, location and nature of glycosylation as well as possible disulfides.

我们目前正在研究一种激活的G蛋白偶联受体