IVEM USER TRAINING

IVEM 用户培训

• 批准号: 6119700
• 负责人: MICHAEL MARKO
• 金额: $ 0.28万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119700
• 关键词: Mammalia; bacteria; bioimaging /biomedical imaging; biomedical equipment development; biomedical resource; computers; human tissue; immunology; infection; inflammation; lymphatic system; microscopy; parasitism

IVEM training is done similar to HVEM training. There are a wide range of systems available on the IVEM, including low-dose semi-automated and fully-automated tomography (using either film or slow-scan CCD recording), low-dose cryo-EM, STEM, and X-ray microanalysis. For ordinary use only a short training period is required, but for special modes and operations one or more days of training are required, depending on the experience of the user. For short projects involving advanced techniques for inexperienced users, the microscope is operated by M. Marko with the user looking on. In all cases, M. Marko or K. Buttle sets up the microscope appropriately for each user, changes the specimens, and develops the film. As with the HVEM, the project is discussed beforehand with the user to ensure that the specimen and microscope conditions will be appropriate for the study at hand. Assistance is also given with interpretation of results and further processing in the cas e of tomo graphy or Sterecon applications. Every opportunity is taken at meetings to advertise the capabilities of our IVEM facility. Extensive documentation on the use of the IVEM for tomography and low-dose imaging was written for the SPIDER www homepage. Both internal users and visitors can review procedures before using the microscope.

IVEM培训与HVEM培训类似。 有各种 IVEM上可用的一系列系统，包括低剂量 半自动和全自动断层扫描（使用胶片或 慢扫描CCD记录）、低剂量冷冻EM、STEM和X射线 微观分析 对于普通使用， 需要，但对于特殊模式和操作， 根据用户的经验，需要进行培训。 为 短期项目涉及先进的技术，为没有经验的用户， 显微镜由M操作。Marko与用户一起观看。在 所有案件，M。马尔科还是K巴特尔适当地设置了显微镜 为每个用户更换样本并冲洗胶片。 如同 HVEM，项目事先与用户讨论，以确保 样品和显微镜条件将适合于 手头的研究。 还提供口译服务， 在断层摄影或Sterecon情况下的结果和进一步处理 应用. 在会议上利用一切机会宣传 我们的IVEM设施的能力。 关于使用的大量文档 IVEM的断层扫描和低剂量成像是为 www.spider.com 内部用户和访问者都可以查看 使用显微镜前的步骤