MOLECULAR CLONING & REGULATION OF HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE GENE

分子克隆

• 批准号: 6122470
• 负责人: DAVID A GELLER
• 金额: --
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6122470
• 关键词: biological products; biomedical resource; computers; genetics

Induced nitric oxide synthase is known to occur in several tissues during sepsis and inflammation. Activation of the nitric oxide pathway has both deleterious and beneficial consequences. At present, no information is available on the transcriptional regulation of human inducible nitric oxide synthase gene expression by cytokines and endotoxin. Our laboratory has recently cloned the human hepatocyte inducible nitric oxide synthase gene(Geller et al. Proc Natl Acad Sci USA 1993; 90:3491-33495). We will use this cDNA to screen a human genomic DNA library and isolate the promoter region of the human induced nitric oxide synthase gene. The promoter will be sequenced and analyzed for transcriptional factor binding sites to characterize the transcriptional regulation of this important enzyme. It is our hope that the information learned by these studies will contribute significantly to our understanding of transcriptional regulation of gene expression in sepsis and, more importantly, the regulation of induced nitric oxide synthesis in sepsis and inflammation.

已知诱导性一氧化氮合酶存在于多种组织中 在败血症和炎症期间。 一氧化氮的活化 途径既有有害的也有有益的后果。 现在， 目前还没有关于人类转录调控的信息 细胞因子诱导一氧化氮合酶基因表达 内毒素。 我们实验室最近克隆了人肝细胞 诱导型一氧化氮合酶基因（Geller et al. Proc Natl Acad Sci 美国 1993； 90:3491-33495）。 我们将使用该 cDNA 来筛选人类 基因组DNA文库并分离人类的启动子区域 诱导一氧化氮合酶基因。 启动子将被测序 并分析转录因子结合位点来表征 这种重要酶的转录调控。 这是我们的 希望通过这些研究获得的信息能够有所贡献 对我们对转录调控的理解具有重要意义 脓毒症中的基因表达，更重要的是， 在脓毒症和炎症中诱导一氧化氮合成。