MOLECULAR CLONING & REGULATION OF HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE GENE

已知诱导型一氧化氮合酶存在于几种组织中 在脓毒症和炎症期间。一氧化氮的激活 路径既有有害的后果，也有有益的后果。目前， 目前还没有关于人类基因转录调控的信息 细胞因子与诱导型一氧化氮合酶基因的表达 内毒素。我们实验室最近克隆了人类肝细胞。 诱导型一氧化氮合酶基因(Geller等人。Proc Natl Acad科学 美国，1993年；90：3491-33495)。我们将用这个cdna来筛选一个人 人基因组DNA文库及启动子区域的分离 诱导型一氧化氮合酶基因。启动子将被测序 并对转录因子结合位点进行分析以表征 这种重要酶的转录调节。这是我们的 希望通过这些研究获得的信息将有助于 对我们理解转录调控有重要意义 脓毒症中的基因表达，更重要的是，对 在脓毒症和炎症中诱导一氧化氮合成。

Induced nitric oxide synthase is known to occur in several tissues during sepsis and inflammation. Activation of the nitric oxide pathway has both deleterious and beneficial consequences. At present, no information is available on the transcriptional regulation of human inducible nitric oxide synthase gene expression by cytokines and endotoxin. Our laboratory has recently cloned the human hepatocyte inducible nitric oxide synthase gene(Geller et al. Proc Natl Acad Sci USA 1993; 90:3491-33495). We will use this cDNA to screen a human genomic DNA library and isolate the promoter region of the human induced nitric oxide synthase gene. The promoter will be sequenced and analyzed for transcriptional factor binding sites to characterize the transcriptional regulation of this important enzyme. It is our hope that the information learned by these studies will contribute significantly to our understanding of transcriptional regulation of gene expression in sepsis and, more importantly, the regulation of induced nitric oxide synthesis in sepsis and inflammation.