MOLECULAR CLONING & REGULATION OF HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE GENE

分子克隆

• 批准号: 6282505
• 负责人: DAVID A GELLER
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282505
• 关键词: biological products; biomedical resource; computers; genetics

Induced nitric oxide synthase is known to occur in several tissues during sepsis and inflammation. Activation of the nitric oxide pathway has both deleterious and beneficial consequences. At present, no information is available on the transcriptional regulation of human inducible nitric oxide synthase gene expression by cytokines and endotoxin. Our laboratory has recently cloned the human hepatocyte inducible nitric oxide synthase gene(Geller et al. Proc Natl Acad Sci USA 1993; 90:3491-33495). We will use this cDNA to screen a human genomic DNA library and isolate the promoter region of the human induced nitric oxide synthase gene. The promoter will be sequenced and analyzed for transcriptional factor binding sites to characterize the transcriptional regulation of this important enzyme. It is our hope that the information learned by these studies will contribute significantly to our understanding of transcriptional regulation of gene expression in sepsis and, more importantly, the regulation of induced nitric oxide synthesis in sepsis and inflammation.

已知诱导型一氧化氮合酶存在于多种组织中 感染败血症和炎症。 激活一氧化氮 这一途径既有有害的后果，也有有益的后果。 目前， 没有关于人类基因组转录调控的信息。 细胞因子诱导型一氧化氮合酶基因表达， 内毒素 我们实验室最近克隆了人类肝细胞 诱导型一氧化氮合酶基因（Geller等，Proc Natl Acad Sci USA 1993; 90：3491-33495）。 我们将使用这个cDNA来筛选人类 基因组DNA文库，并分离人 诱导型一氧化氮合酶基因 启动子将被测序 并分析转录因子结合位点以表征 这一重要酶的转录调控。 是我们 我希望通过这些研究获得的信息将有助于 对我们理解转录调控有重要意义， 脓毒症中的基因表达，更重要的是， 诱导脓毒症和炎症中的一氧化氮合成。