MOLECULAR CLONING & REGULATION OF HUMAN INDUCIBLE NITRIC OXIDE SYNTHASE GENE

分子克隆

• 批准号: 6295160
• 负责人: DAVID A GELLER
• 金额: $ 1.19万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295160
• 关键词: biological products; biomedical resource; computers; genetics

Induced nitric oxide synthase is known to occur in several tissues during sepsis and inflammation. Activation of the nitric oxide pathway has both deleterious and beneficial consequences. At present, no information is available on the transcriptional regulation of human inducible nitric oxide synthase gene expression by cytokines and endotoxin. Our laboratory has recently cloned the human hepatocyte inducible nitric oxide synthase gene(Geller et al. Proc Natl Acad Sci USA 1993; 90:3491-33495). We will use this cDNA to screen a human genomic DNA library and isolate the promoter region of the human induced nitric oxide synthase gene. The promoter will be sequenced and analyzed for transcriptional factor binding sites to characterize the transcriptional regulation of this important enzyme. It is our hope that the information learned by these studies will contribute significantly to our understanding of transcriptional regulation of gene expression in sepsis and, more importantly, the regulation of induced nitric oxide synthesis in sepsis and inflammation.

诱导型一氧化氮合酶已知存在于几种组织中