FOLDING OF INFLUENZA VIRUS GENES

流感病毒基因折叠

• 批准号: 6295112
• 负责人: M LOUISE HERLOCHER
• 金额: $ 1.17万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295112
• 关键词: biological products; biomedical resource; genetics; growth /development; infection; virus

All eight genes of the original isolate of wild type A/AA/6/60 influenza virus and of the cold adapted A/AA/6/60 vaccine virus have been cloned, sequenced and subjected to RNAfold analysis. Both viruses are ts and attenuated for ferrets. To complete the study, a wild type of the same virus which is both non-ts and virulent will be sequenced and subjected to RNAfold analysis. Relevance of the RNAfolds to the phenotypes of temperature sensitivity and cold adaption as well as to transport of vRNA out of the nucleus will be explored. The second objective of this series of studies is to compare computer generated RNAfold predictions to experimentally deduced structures. SJCRH has a database of 92 nonstructural gene sequences (the smallest gene - 890 bases - of the influenza virus). I propose to study their predicted RNAfolds using the Zuker algorithm on the Y-MP to determine whether the folds of the NS gene will fall into groups corresponding to host of origin and whether conserved domains necessary for functions such as exodus from the cell nucleus may be identified. I will then compare the computer predicted folds to experimentally deduced structures using mung bean nuclease and psoralen cross-linking studies.

野生型A/AA/6/60原始分离株的所有8个基因 流感病毒和冷适应的A/AA/6/60疫苗病毒已经 克隆、测序并进行RNA折叠分析。 两 对于雪貂来说，病毒是ts病毒，并且是减毒的。 为了完成这项研究， 非ts和毒性的相同病毒的野生型将被 测序并进行RNA折叠分析。 相关性 RNA折叠对温度敏感性和寒冷表型的影响 适应以及将vRNA运输出细胞核将是 探讨了 这一系列研究的第二个目标是 将计算机生成的RNA折叠预测与实验进行比较 推断结构。 SJCRH拥有92个非结构基因的数据库 序列（最小的基因- 890个碱基-流感病毒）。 我 建议使用Zuker算法研究他们预测的RNAfolds， Y-MP来确定NS基因的折叠是否会落入 组对应的主机的起源和是否保守域 对于诸如从细胞核出窍的功能所必需的， 鉴定 然后，我将比较计算机预测的褶皱， 使用绿豆核酸酶实验推导的结构， peppen交联研究。