FOLDING OF INFLUENZA VIRUS GENES

流感病毒基因折叠

• 批准号: 6282457
• 负责人: M LOUISE HERLOCHER
• 金额: $ 1.17万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6282457
• 关键词: biological products; biomedical resource; genetics; growth /development; infection; virus

All eight genes of the original isolate of wild type A/AA/6/60 influenza virus and of the cold adapted A/AA/6/60 vaccine virus have been cloned, sequenced and subjected to RNAfold analysis. Both viruses are ts and attenuated for ferrets. To complete the study, a wild type of the same virus which is both non-ts and virulent will be sequenced and subjected to RNAfold analysis. Relevance of the RNAfolds to the phenotypes of temperature sensitivity and cold adaption as well as to transport of vRNA out of the nucleus will be explored. The second objective of this series of studies is to compare computer generated RNAfold predictions to experimentally deduced structures. SJCRH has a database of 92 nonstructural gene sequences (the smallest gene - 890 bases - of the influenza virus). I propose to study their predicted RNAfolds using the Zuker algorithm on the Y-MP to determine whether the folds of the NS gene will fall into groups corresponding to host of origin and whether conserved domains necessary for functions such as exodus from the cell nucleus may be identified. I will then compare the computer predicted folds to experimentally deduced structures using mung bean nuclease and psoralen cross-linking studies.

野生型 A/AA/6/60 原始分离株的所有八个基因 流感病毒和冷适应A/AA/6/60疫苗病毒有 被克隆、测序并进行 RNAfold 分析。 两个都 病毒对雪貂来说是 ts 和减毒的。 为了完成这项研究， 既非 ts 又具有毒性的同一病毒的野生型将 测序并进行 RNAfold 分析。 相关性 RNAfolds 对温度敏感和冷的表型 适应以及将 vRNA 运输出细胞核将 探索过。 这一系列研究的第二个目标是 将计算机生成的 RNAfold 预测与实验结果进行比较 推导的结构。 SJCRH拥有92个非结构基因数据库 序列（流感病毒的最小基因 - 890 个碱基）。 我 提议使用 Zuker 算法研究他们预测的 RNAfolds Y-MP来确定NS基因的折叠是否会落入 对应于来源主机的组以及是否保守域 诸如从细胞核中流出等功能所必需的可能是 确定。 然后我会将计算机预测的折叠与 使用绿豆核酸酶实验推导的结构 补骨脂素交联研究。