FOLDING OF INFLUENZA VIRUS GENES

伽马射线照射的 D2XRII 小鼠细胞的共培养 骨髓基质细胞系 Interlcukin-3/粒细胞巨噬细胞集落刺激因子 (GM-CSF)依赖性造血祖细胞系FDC-PlJL26 刺激因子独立造血细胞的出现 子线。 多项证据表明 M-CSF 或蛋白质 与 D2XRII 细胞表达的 M-CSF (LSF) 具有抗原相关性，可能 在独立因素的出现中发挥了作用 子线。 为了分离出与抗原相关的因子 M-CSF，分子克隆是从 D2XRII cDNA 文库中分离出来的 与小鼠 M-CSF 基因探针杂交。 其中两个分子 克隆，命名为 60.8.2 和 6452。包含 885 个碱基对 (bp) M-CSF 编码区缺失。 这样的 cDNA 克隆尚未被 先前在小鼠中描述过，但有与其同源的 cDNA 克隆 已从人胰腺肿瘤细胞系 MIA-PaCa-2 中分离出来。 检测到三个转录本（4.8 Kb、3.4 kb 和 1.8kb） 与 RNA 转录本特异的寡核苷酸杂交 包含 60.8.2 删除。 这些被删除的文字记录并没有 似乎是由电离辐射诱导的；然而，另外两种 M-CSF 大小为 4.4 kb 和 2.3 kb 的转录本显示积累增加 伽马射线照射后。 逆转录聚合酶链 反应 (RT-PCR) 显示删除特定转录本 (8) 不仅在 D2XRII 细胞中而且在几种小鼠骨骼中都检测到 骨髓基质细胞系和几种小鼠组织中。

Cocultivation of cells from the gamma irradiated D2XRII murine bone marrow stromal cell line with Interlcukin-3/granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent hematopoietic progenitor cell line FDC-PlJL26 stimulates the emergence of factor independent hematopoietic cell sublines. Several lines of evidence suggested that M-CSF or a protein antigenetically related to M-CSF (LSF) expressed by D2XRII cells, may have played a role in the emergence of the factor independent sublines. In an effort to isolate a factor antigenetically related to M-CSF, molecular clones were isolated from a D2XRII cDNA library that hybridized to a mouse M-CSF, genetic probe. Two of these molecular clones, designated 60.8.2 and 6452. contained an 885 base-pari (bp) deletion in the M-CSF coding region. Such a cDNA clone has not been previously described in the mouse but a cDNA clone homologous to it has been isolated from a human pancreatic tumor cell line, MIA-PaCa-2. Three tra nscripts (4.8 Kb, 3.4 kb, and 1.8kb) were detected that hybridized to an oligonucleotide that was specific to RNA transcripts containing the 60.8.2 deletion. These deleted transcripts do not appear to be induced by ionizing irradiation; however, two other M-CSF transcripts of sizes to 4.4 kb and 2.3 kb show increased accumulation after gamma irradiation. Reverse transcription-polymerase chain reaction (RT-PCR) revealed that the deletion specific transcript (8) were detected not only in D2XRII cells but also in several mouse bone marrow stromal cell lines and in several mouse tissues.