COMPUTER ANALYSIS OF PRIMARY STRUCT OF DVMTLY REGULATED PROTEIN TYROSINE KINASES

DVMT调节蛋白酪氨酸激酶一级结构的计算机分析

• 批准号: 6295107
• 负责人: DAVID R BURGESS
• 金额: $ 1.17万
• 依托单位: MELLON PITTS CORPORATION (MPC CORP)
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6295107
• 关键词: animal tissue; biomedical resource; growth /development; intestines

It has been shown by several labs including our own, that development of cells involves differential activity of certain protein tyrosine kinases. In order to study this further we are attempting to clone genes encoding tyrosine kinases from a cDNA library using immunological probes. Once the clones are isolated it will be necessary to identify them through sequence analysis. The sequences will then be compared to known sequences using existing libraries such as Genbank, etc. Using the sequence information thus acquired along with biochemical data about the activity of the proteins in cells in various states of differentiation, it should be possible to make some conclusions about the role(s) of the isolated gene products during development.

包括我们自己在内的几个实验室已经证明， 细胞的发育涉及某些蛋白质的不同活性 酪氨酸激酶。为了进一步研究这一点，我们正在尝试 从文库中克隆编码酪氨酸激酶的基因 免疫探头。一旦克隆被隔离，它将被 有必要通过序列分析来鉴定它们。这些序列 然后与使用现有文库的已知序列进行比较，如 作为Genbank等。使用这样获得的序列信息 有了关于细胞中蛋白质活性的生化数据 不同的分化状态，应该有可能做出一些 关于分离的基因产物在过程中的作用(S)的结论 发展。