State-of-the-art cell pulsatile physiological stretch & live cell imaging system

最先进的细胞脉动生理拉伸

• 批准号: MR/X013820/1
• 负责人: Richard Coward
• 金额: $ 13.57万
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX013820%2F1
• 关键词: State; art; cell; pulsatile; physiological

Studying cells in an environment that resembles normal physiology experienced in the body is desirable. In the University of Bristol we have multiple research groups who are interested in kidney, cardiovascular, tissue engineering and cancer cell/organoid biology. We have identified that growing cells in a system that allows dynamic pulsatile stretch that would mimic cardiac output (amplitude and frequency) would be highly beneficial for these groups.We would like to purchase a state-of-the-art stretch system that can simultaneously stretch multiple cell/organoid/tissue samples in an incubator for days to weeks (matching the situation in the body). Furthermore, it has the capability to video the specimens through an incubator located microscope and assess how proteins change in their cellular location through florescent tags.This equipment will be made available to all researchers in the University by being linked to our dedicated Wolfson Imaging Centre. Importantly, there is currently no other equipment that can do this in our University, so this equipment would facilitate an unmet need.

在与人体经历的正常生理相似的环境中研究细胞是可取的。在布里斯托尔大学，我们有多个研究小组，他们对肾脏、心血管、组织工程和癌细胞/有机物生物学感兴趣。我们已经确定，在一个允许动态脉动拉伸的系统中培养细胞将对这些群体非常有利。我们想购买一种最先进的拉伸系统，可以在孵化器中同时拉伸多个细胞/器官/组织样本数天到数周(与体内的情况相匹配)。此外，它还能够通过位于孵化器的显微镜拍摄样本，并通过荧光标记评估蛋白质在细胞位置上的变化。这台设备将通过连接到我们专门的沃尔夫森成像中心向大学的所有研究人员提供。重要的是，目前我们大学还没有其他设备可以做到这一点，所以这种设备将满足未得到满足的需求。