ENCODING IN SOMATIC SENSE ORGANS

躯体感觉器官的编码

• 批准号: 6139567
• 负责人: CARLTON C HUNT
• 金额: $ 12.48万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6139567
• 关键词: action potentials; antidromic impulse; biological signal transduction; cats; confocal scanning microscopy; electrostimulus; fluorescent dye /probe; image processing; mechanoreceptors; nerve endings; neural conduction; neural information processing; neuromuscular function; photochemistry; sensory mechanism; sodium channel; somatic afferent nerve; spinal ganglion; voltage gated channel

DESCRIPTION (Adapted from the applicant's abstract): The long term objective of this work is to understand the mechanisms of sensory transduction in mammalian somatic receptors that sense mechanical changes. Several steps are involved in this process: transmission of the mechanical signal to the sensory terminals of the primary afferent neuron, conductance changes in the terminals-leading to the receptor potential, its electrotonic spread to the site of impulse-initiation and propagation of action potentials in the sensory axon. While the impulse train in response to a mechanical stimulus, i.e. the end result of the sensory transduction, is well known, there are very few data on the several steps leading to the sensory message sent to the C.N.S. This proposal is for study of impulse-initiation in preparations of several mechanoreceptors which can be studied in isolation. Using microscopic visualization with image processing and high speed photodetectors to record rapid changes, electrical potential changes in different regions of the sensory endings will be detected by voltage- sensitive fluorescent probes. Electrical recording with micropipettes would also be used. The site, or sites of impulse initiation, as well as their subsequent propagation, will be studied in Pacinian corpuscles and in muscle spindles. Are there multiple pacemaker sites in receptors supplied by several branches of the sensory axon? Is there propagation antidromically toward the sensory terminals? If so, how does this affect the spread of the receptor potential and the consequent sensory impulse train sent to the C.N.S? This information, in an area which has been barely explored, could greatly improve our understanding of how mechanical events are sensed. This could open up new areas of inquiry of possible importance in sensory deficits.

描述（改编自申请人摘要）： 这项工作的长期目标是了解 哺乳动物体细胞感受器的感觉传导 机械变化。这一过程涉及几个步骤： 将机械信号传输到神经元的感觉终端， 初级传入神经元，终末传导变化，传导通路 到受体电位，其电紧张性扩散到 感觉动作电位的脉冲起始和传播 轴突 而响应于机械刺激的脉冲序列，即 感官传导的最终结果，是众所周知的，有非常 几个步骤上的一些数据，导致感官信息发送到 C.N.S.这一建议是为研究脉冲引发制剂 几种机械感受器，可以单独研究。使用 具有图像处理和高速的显微可视化 光电探测器记录快速变化， 感觉末梢的不同区域将被电压检测到- 灵敏的荧光探针。用微量移液器进行电记录 也将被使用。冲动产生的部位 作为其随后的传播，将在Pacinian小体中进行研究 和肌梭中。受体中是否存在多个起搏点 是由感觉轴突的几个分支提供的有传播吗 逆向地向感觉末梢运动如果是这样， 感受器电位的扩散和随之而来的感觉冲动 送去国安局的火车吗这些信息，在一个已经被 几乎没有被探索过，可以大大提高我们对 感测机械事件。这可能会开辟新的研究领域 可能对感官缺陷有重要影响