ENCODING IN SOMATIC SENSE ORGANS

描述(改编自申请人的摘要)： 这项工作的长期目标是了解 哺乳动物体细胞感受器的感觉转导 机械上的变化。此过程涉及以下几个步骤： 将机械信号传输到机器人的感官终端 初级传入神经元，终末电导变化--导联 到感受器电位，它的电张力扩散到 动作电位在感觉器官中的冲动引发和传播 轴突。 而脉冲序列响应于机械刺激，即 感官传导的最终结果，是众所周知的，有很多 几乎没有关于导致感官信息发送到 这项建议是为了研究制剂中的脉冲引发。 几个可以单独研究的机械感受器。vbl.使用 基于图像处理和高速的显微可视化 光电探测器记录快速变化、电势变化 感官末梢的不同区域将通过电压检测- 灵敏的荧光探针。用微吸管进行电记录 也会被使用。一个或多个冲动起始点，以及 作为它们随后的繁殖，将在太平洋小体中进行研究 以及肌梭。受体中是否有多个起搏器部位？ 由感觉神经轴突的几个分支提供？有没有传播 逆着方向朝向感官终端？如果是这样的话，这会有什么影响 感受器电势的扩散和由此产生的感觉冲动 火车被送到了国安局？这一信息，在一个已经被 几乎没有被探索过，可以极大地提高我们对 机械事件是可以感知到的。这可能会开辟新的调查领域 可能对感官缺陷有重要影响。

DESCRIPTION (Adapted from the applicant's abstract): The long term objective of this work is to understand the mechanisms of sensory transduction in mammalian somatic receptors that sense mechanical changes. Several steps are involved in this process: transmission of the mechanical signal to the sensory terminals of the primary afferent neuron, conductance changes in the terminals-leading to the receptor potential, its electrotonic spread to the site of impulse-initiation and propagation of action potentials in the sensory axon. While the impulse train in response to a mechanical stimulus, i.e. the end result of the sensory transduction, is well known, there are very few data on the several steps leading to the sensory message sent to the C.N.S. This proposal is for study of impulse-initiation in preparations of several mechanoreceptors which can be studied in isolation. Using microscopic visualization with image processing and high speed photodetectors to record rapid changes, electrical potential changes in different regions of the sensory endings will be detected by voltage- sensitive fluorescent probes. Electrical recording with micropipettes would also be used. The site, or sites of impulse initiation, as well as their subsequent propagation, will be studied in Pacinian corpuscles and in muscle spindles. Are there multiple pacemaker sites in receptors supplied by several branches of the sensory axon? Is there propagation antidromically toward the sensory terminals? If so, how does this affect the spread of the receptor potential and the consequent sensory impulse train sent to the C.N.S? This information, in an area which has been barely explored, could greatly improve our understanding of how mechanical events are sensed. This could open up new areas of inquiry of possible importance in sensory deficits.