SCREENING POTENTIAL CHEMOPREVENTIVE AGENTS IN MICE

在小鼠中筛选潜在的化学预防剂

• 批准号: 6326828
• 负责人: Clinton Julian Grubbs
• 金额: --
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-30 至 2001-06-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6326828
• 关键词: adenomatous polyps; animal breeding; cancer prevention; carcinogenesis inhibitor; chemoprevention; colorectal neoplasms; disease /disorder model; drug screening /evaluation; gene mutation; laboratory mouse; model design /development; neoplasm /cancer genetics

The primary objective of this workstatement is to investigate the chemopreventive efficacy of a variety of agents employing transgenic animals which have mutations similar to those found in humans and which spontaneously develop colon tumors. The first mouse model (Min mouse) has a mutation in the APC gene and develops multiple adenomas in a manner analogous to human FAP (familial adnomatous polyposis) patients. These mice will be employed to screen for four different potential chemopreventive agents. In addition two highly effective aspects will be employed to determine whether these agents are still effective one early, pre invasive, adenomas have arisen. The second animal model has a deficiency in the repair gene MSH-2 and appear comparable to humans with HNPCC (hereditary nonpolyposis colon cancer). These mice will be bred containing both APC and MSH-2 mutations will be bred and will be examined for chemopreventive efficacy employing a variety of chemopreventive agents. The results of these animal studies, employing these directly relevant models, are likely to be immediately applicable in determining agents for potential human trials.

本工作说明的主要目的是调查