GOAT MODEL OF EHRLICHIA CHAFFEENSIS RESERVOIR INFECTION

恰芬埃里希体水库感染的山羊模型

基本信息

批准号：
6091748
负责人：
SUSAN E LITTLE
金额：
$ 13.91万
依托单位：
UNIVERSITY OF GEORGIA
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-04-01 至 2004-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6091748
关键词：
Ehrlichia Ungulate bacteria infection mechanism disease /disorder model disease reservoirs ehrlichiosis genetic strain goats immunologic memory microorganism immunology model design /development ticks

项目摘要

Our long range goal is to understand how E. chafeensis and other ehrlichial organisms are maintained in nature. As a major objective in pursuit of this goal, this application proposes to develop the domestic goal as a model of E. chafeensis vertebrate infection. The central hypothesis is that domestic goats can serve as a much-needed surrogate to white-tailed deer in laboratory based studies of E. chafeensis reservoir infection dynamics. The rationale behind the proposed research is that goats could provide a superior laboratory model of reservoir host infection to white-tailed deer. Goats have a much more tractable nature than deer and they remain easy to handle throughout their life. This docile nature allows greater latitude in the design of infection studies and reduces the risk of handling-associated injury. In addition, goats are more widely available than deer for research, a higher degree of similarity can be obtained between experimental animals due to controlled inbreeding, they are easily raised in tick-free (and thus Ehrlichia-exposure free) facilities, and a larger variety of laboratory reagents are available for work with goats. In short, a goat model of vertebrate reservoir infection, once developed, would allow design and conduct of experiments not readily possible with deer. To accomplish the objective of this application, we will pursue four specific aims: (1) determine how E. chafeensis infection develops and is maintained in domestic goats, (2) evaluates the persistence of E. chafeensis infection in the absence of re-exposure, domestic goats, (2) evaluate the persistence of E. chafeensis infection in the absence of re-exposure, (3) evaluate the response of goats to multiple re-challenge with a homologous strain of E. chafeensis, and (4) transmit E. chafeensis from infected to naive domestic goats via tick feeding. At the completion of this research we expect to have developed an alternative ruminant model for studying E. chafeensis within the vertebrate reservoir host by characterizing the course of E. chafeensis infection within the domestic goal. Additionally, we will have evaluated both the persistence of infection in goats in the absence of re-exposure and the response of goats to multiple, frequent re-exposure, as well as have developed a natural model of infection in goats via tick feeding. The development of this model and its subsequent application in future experiments will facilitate understanding about the dynamics of E. chafeensis infection in the natural wild reservoir host. New control strategies could result.

我们的远距离目标是了解大肠杆菌和其他ehrlichial生物在自然界中如何保持。作为追求这一目标的主要目标，该申请提议将国内目标发展为Chafeensis脊椎动物感染的模型。中心假设是，在基于实验室的Chafeensis储层感染动力学研究中，国内山羊可以成为急需的白尾鹿的替代物。拟议的研究背后的理由是，山羊可以为白尾鹿提供储层宿主感染的优质实验室模型。山羊的性质比鹿具有更高的性质，并且它们一生都很容易处理。这种温顺的性质可以在感染研究的设计中更大的纬度，并降低处理相关损伤的风险。此外，山羊比研究更广泛地可用，由于受控的近交，可以在实验动物之间获得更高程度的相似性，它们很容易在不含tick的无滴答性（因此无ehrlichia-tebo-timposure）的设施中饲养，并且可以使用较大的实验室试剂可用于与山羊一起工作。简而言之，一旦开发出脊椎动物储层感染的山羊模型，将允许使用鹿的设计和进行实验。为了实现此应用的目标，我们将追求四个具体目的：（1）确定Chafeensis感染如何发展并保持在国内山羊中，（2）评估Chafeensis感染的持续性，缺乏重新曝光，家用山羊，（2）评估Chafeensis Intivection Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive Intive the Re-Re-Re-Re-Re-Re-Intive（2）与Chafeensis的同源菌株重新挑战，（4）通过tick喂食将大肠杆菌从感染到天真的家山羊转移。这项研究完成后，我们希望通过表征国内目标中的Chafeensis感染过程来开发一种替代的反刍模型，用于研究脊椎动物储层宿主内的Chafeensis。此外，我们将在没有重新曝光的情况下评估山羊感染的持续性和山羊对多个频繁重新暴露的反应，并通过tick喂食在山羊中开发了自然的感染模型。该模型的开发及其在未来的实验中的后续应用将有助于了解天然野生储层宿主中chafeensis感染的动态。可能会导致新的控制策略。