ROLE OF NEUROPEPTIDES AND BIOGENIC AMINES IN BRAIN FUNCTION

我们继续研究血管紧张素II和其他神经肽， 生物胺受体 我们克隆了一种新的血管紧张素II受体， 高度定位于沙鼠的海马体，可能 在神经可塑性中的作用。 分子研究揭示了 可能决定受体抑制剂的亲和力。 使用受体 缺陷小鼠，我们发现脑血管紧张素的表达， 受体亚型不仅依赖于大脑区域，但在某些情况下， 研究的物种。 我们发现，在背弓状核， 血管紧张素AT1受体。 这些受体被上调 雌激素和孕激素在女性，参与分泌 它们是由多巴胺能神经元产生的。 因此我们 首次显示了血管紧张素和 脑中的儿茶酚胺系统 我们完成了AT1受体表达和mRNA的定位， 和AT2血管紧张素受体亚型， 并获得了指示AT2受体通过以下途径转运的数据 从下橄榄到小脑皮质的攀爬纤维

We continued our studies on angiotensin II and other neuropeptide and biogenic amine receptors. We cloned a new angiotensin II receptor, highly localized to the hippocampus of the gerbil, with possible function in neuronal plasticity. Molecular studies revealed the domain likely to determine affinity for receptor inhibitors. Using receptor deficient mice, we found that the expression of brain angiotensin receptor subtypes was dependent not only on the brain area, but, in some areas, on the species studied. We identified, in the dorsal arcuate nucleus, a sexually dimorphic area for angiotensin AT1 receptors. These receptors are up-regulated by estrogen and progesterone in the female, are involved in the secretion of prolactin, and they are made by dopaminergic neurons. Thus, we showed for the first time a cellular linkage between the angiotensin and catecholamine systems in the brain. We completed the localization of receptor expression and mRNA for AT1 and AT2 angiotensin receptor subtypes in the olivo-cerebellar pathway, and obtained data indicative of a transport of AT2 receptors via climbing fibers from the inferior olive to the cerebellar cortex.