ROLE OF NEUROPEPTIDES AND BIOGENIC AMINES IN NEUROENDOCRINE REGULATION

褪黑激素受体：我们表征了高亲和力褪黑激素受体， 大脑核团以及大脑和尾动脉中涉及温度 这些受体与鸟嘌呤核苷酸结合蛋白偶联。 褪黑激素调节尾动脉收缩。 血管紧张素受体：脑血管紧张素受体更广泛地 在发育中的大鼠比在成熟的动物中表达， 选择性地由特定的离子缺陷和细胞外的 流体体积。受体可以使用放射自显影进行分类， 用新的特异性受体抑制剂取代。 β-肾上腺素受体：β（2）-肾上腺素受体控制调节 皮质5-羟色胺2周围交感神经节含有 只有β（2）-肾上腺素受体，受体数量在遗传上很高， 高血压大鼠 方法：我们开发了新的敏感的放射自显影放射免疫- 组织抗原定量的组织化学方法 （神经肽，肽酶，胺受体）在选定的大脑区域，和 在人类血小板中。这些方法的灵敏度比其他方法高50倍以上。 经典的方法，并将允许新的药理学和临床研究。

Melatonin receptors: We characterized high affinity melatonin receptors in cerebral nuclei and in brain and caudal arteries involved in temperature control These receptors are coupled to guanine nucleotide binding proteins. Melatonin modulates caudal artery contractions. Angiotensin receptors: Brain angiotensin receptors are more widely expressed in developing rats than in mature animals, and they are selectively modulated by specific ion deficiencies and by the extracellular fluid volume. The receptors can be classified using autoradiography and displacement with new specific receptor inhibitors. Beta-adrenoceptors: Beta(2)-adrenoceptors control the regulation of cortical serotonin 2 during stress. Peripheral sympathetic ganglia contain only Beta(2)-adrenoceptors, and the receptor number is high in genetically hypertensive rats. Methodology: We developed new sensitive autoradiographic radioimmuno-- histochemical methods for the quantification of tissue antigens (neuropeptides, peptidases, amine receptors) in selected brain areas, and in human platelets. These methods have higher-over 50 fold-sensitivity than classical methods and will allow new pharmacological and clinical studies.