CRANIOFACIAL DEVELOPMENT

颅面发育

• 批准号: 6176667
• 负责人: GURKIRPAL S SOHAL
• 金额: $ 25.01万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6176667
• 关键词: Retroviridae; antisense nucleic acid; bone development; cadherins; cell membrane; cell migration; cell type; chick embryo; craniofacial; extracellular matrix; fluorescent dye /probe; histogenesis; immunocytochemistry; integrins; microinjections; microsurgery; neural crest; neural plate /tube; rhombencephalon; transfection /expression vector; trigeminal nerve

The long-term goal of this research is to expand our understanding of the biological basis of craniofacial morphogensesis and the underlying causes of craniofacial anomalies. The main goal of the proposed studies is to understand the contributions of a newly described cell type in craniofacial development. We have recently reported that well, after the emigration of neural crest cells has finished, and additional population of cells emigrates from the neural tube. In contrast to the neural crest, this population originates in the ventral portion of the hindbrain neural tube, emigrates at the point of attachment of the trigeminal nerve, and migrates into craniofacial structures. This had led us to hypothesize that these ventral neural tube cells contribute to the formation of craniofacial tissues. The proposed studies will investigate the precise origin of these cells, the molecules that guide their migration, and their contributions. Neural tube cells of chick embryos will be labeled with fluorescent dye and replication-deficient retroviruses to follow their migration and determine their fate. Molecules that guide their migration will be investigated by immunocytochemical detection of cell surface and extracelluar matrix molecules and by antisense strategy. The importance of this population of cell in craniofacial morphogenesis will be investigated by microsurgical interventions. Our current understanding of craniofacial morphogenesis and malformations is based on the belief that the neural crest is the sole neural tube-derived population that migrates into craniofacial structures. The present study will reveal the contributions of a second neural tube-derived population. Results of this study likely will alter our interpretations of mechanisms of normal development, and of the underlying causes of craniofacial anomalies, which make up about 25 percent of all malformations.

这项研究的长期目标是扩大我们对 颅面形态发生的生物学基础及其潜在的 颅面畸形的原因 拟议研究的主要目标 是了解一种新描述的细胞类型在 颅面发育我们最近报道说， 神经嵴细胞的迁移已经完成， 细胞从神经管中迁移出来。与神经系统相比， 嵴，这个种群起源于腹侧部分的 后脑神经管，在连接点迁移 三叉神经，并迁移到颅面结构。 这已经 让我们假设这些腹侧神经管细胞 颅面组织的形成 拟议的研究将 研究这些细胞的确切起源， 他们的迁徙和贡献 鸡神经管细胞 胚胎将被标记荧光染料和复制缺陷 逆转录病毒跟踪它们的迁移并决定它们的命运。 引导它们迁移的分子将通过以下方法进行研究： 细胞表面和细胞外基质免疫细胞化学检测 分子和反义策略。 这个群体的重要性 细胞在颅面形态发生中的作用将通过 显微外科手术 我们目前对颅面的理解 形态发生和畸形是基于这样的信念，即神经系统 冠是唯一的神经管衍生的人口，迁移到 颅面结构 本研究将揭示 第二神经管衍生群体的贡献。 结果 这项研究很可能会改变我们对正常免疫机制的解释， 发展，以及颅面异常的根本原因， 占所有畸形的25%