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CRANIOFACIAL DEVELOPMENT

颅面发育

基本信息

批准号：
6379826
负责人：
GURKIRPAL S SOHAL
金额：
$ 25.76万
依托单位：
AUGUSTA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-04-01 至 2003-03-31
项目状态：
已结题

项目摘要

The long-term goal of this research is to expand our understanding of the biological basis of craniofacial morphogensesis and the underlying causes of craniofacial anomalies. The main goal of the proposed studies is to understand the contributions of a newly described cell type in craniofacial development. We have recently reported that well, after the emigration of neural crest cells has finished, and additional population of cells emigrates from the neural tube. In contrast to the neural crest, this population originates in the ventral portion of the hindbrain neural tube, emigrates at the point of attachment of the trigeminal nerve, and migrates into craniofacial structures. This had led us to hypothesize that these ventral neural tube cells contribute to the formation of craniofacial tissues. The proposed studies will investigate the precise origin of these cells, the molecules that guide their migration, and their contributions. Neural tube cells of chick embryos will be labeled with fluorescent dye and replication-deficient retroviruses to follow their migration and determine their fate. Molecules that guide their migration will be investigated by immunocytochemical detection of cell surface and extracelluar matrix molecules and by antisense strategy. The importance of this population of cell in craniofacial morphogenesis will be investigated by microsurgical interventions. Our current understanding of craniofacial morphogenesis and malformations is based on the belief that the neural crest is the sole neural tube-derived population that migrates into craniofacial structures. The present study will reveal the contributions of a second neural tube-derived population. Results of this study likely will alter our interpretations of mechanisms of normal development, and of the underlying causes of craniofacial anomalies, which make up about 25 percent of all malformations.

这项研究的长期目标是扩大我们对颅面形态发生的生物学基础及其潜在的颅面畸形的原因拟议研究的主要目标是了解一种新描述的细胞类型在颅面发育我们最近报道说，神经嵴细胞的迁移已经完成，细胞从神经管中迁移出来。与神经系统相比，嵴，这个种群起源于腹侧部分的后脑神经管，在连接点迁移三叉神经，并迁移到颅面结构。这已经让我们假设这些腹侧神经管细胞颅面组织的形成拟议的研究将研究这些细胞的确切起源，他们的迁徙和贡献鸡神经管细胞胚胎将被标记荧光染料和复制缺陷逆转录病毒跟踪它们的迁移并决定它们的命运。引导它们迁移的分子将通过以下方法进行研究：细胞表面和细胞外基质免疫细胞化学检测分子和反义策略。这一群体的重要性细胞在颅面形态发生中的作用将通过显微外科手术我们目前对颅面的理解形态发生和畸形是基于这样的信念，即神经系统冠是唯一的神经管衍生的人口，迁移到颅面结构本研究将揭示第二神经管衍生群体的贡献。结果这项研究很可能会改变我们对正常免疫机制的解释，发展，以及颅面异常的根本原因，占所有畸形的25%