BRAINSTEM RESPIRATORY NEURON INTERACTIONS

这个项目的目标是定义脑干网络机制 高碳酸血症，颈动脉化学感受器，颈动脉 压力感受器和皮肤伤害感受器形成呼吸运动 格局多阵列记录技术，尖峰序列分析， 细胞内记录的尖峰触发平均将用于 测量许多神经元的反应和功能联系， 同时在延髓中缝核，脑桥呼吸组， 和Botzinger腹侧呼吸组（BOT-VRG）， 去脑人工通气猫。网络模型来源于 这些数据将通过计算机模拟进行测试。计划中的实验 将提出五个假设。 假设1：脑桥和延髓网络共同运作， 调节呼吸运动模式以响应：a）脑干 高碳酸血症（即，中枢化学感受器）; B）颈动脉化学感受器 刺激，c）颈动脉压力感受器刺激，和d）皮肤 伤害感受器刺激 假设2：多个反射系统共享脑桥延髓网络， 呼吸运动模式的调节。 假设3：脑桥延髓网络包括内部检查和 调节颈动脉收缩持续时间和幅度的平衡 化学感受器和压力感受器对呼吸的反射作用。 假设4：脑桥延髓网络中的压力感受器反射作用 调节对呼吸的伤害性反射作用。 假设5：反射诱导的瞬态和长期配置 产生的时空模式 同步性，在传统的点火率测量中不明显， 对BOT-VRG进行操作。 改善对呼吸系统疾病的治疗和管理， 心血管疾病的控制依赖于对神经系统 这是一个规范这些功能的系统。该项目将补充 目前正在取得的进展与其他体内和体外 简化方法，并确定网络站点和流程， 遗传或后天的异常可能会扰乱呼吸。

The goal of this project is to define brain stem network mechanisms through which hypercapnia, carotid chemoreceptors, carotid baroreceptors, and cutaneous nociceptors shape the respiratory motor pattern. Multi-array recording technology, spike train analysis, and spike-triggered averaging of intracellular recordings will be used to measure the responses and functional associations of many neurons corded simultaneously in the medullary raphe nuclei, pontine respiratory group, and Botzinger-ventral respiratory group (BOT-VRG) of anesthetized or decerebrate artificially ventilated cats. Network models derived from these data will be tested with computer simulations. Planned experiments will address five hypotheses. Hypothesis 1; Pontine and medullary networks operate together to modulate the respiratory motor pattern in response to: a) brain stem hypercapnia (i.e., central chemoreception); b) carotid chemoreceptor stimulation, c) carotid baroreceptor stimulation, and d) cutaneous nociceptor stimulation. Hypothesis 2: Multiple reflex systems share pontomedullary networks in the regulation of the respiratory motor pattern. Hypothesis 3: Pontomedullary networks include internal checks and balances that regulate the duration and magnitude of carotid chemoreceptor and baroreceptor reflex actions on breathing. Hypothesis 4: Baroreceptor reflex actions in pontomedullary networks modulate nociceptive reflex actions on breathing. Hypothesis 5: Reflexively induced transient and long-term configurations of pontomedullary networks generate spatiotemporal patterns of synchrony, not apparent in conventional measures of firing rate, that act on the BOT-VRG. Improved treatments and management of disorders of respiratory and cardiovascular control depend on improved understanding of the neural system that regulates these functions. This project will complement advances currently being made with other in vivo and in vitro reductionistic approaches and identify network sites and processes where inherited or acquired abnormalities could disrupt breathing.