RAMAN SPECTROSCOPIC EVIDENCE FOR Z DNA IN RAD ENZYME BOUND DNA

其目标是发展表面增强拉曼散射 (SERS)作为一种超灵敏的分析工具在化学、生物学和 医药。SERS效应结合了荧光的敏感性 高结构信息含量的拉曼光谱 光谱学，可用于单分子检测和 身份证明。最近，我们已经实现了单分子检测 并用特殊的方法鉴定“非吸收”分子 制备了胶体团簇和非共振激发。极 获得了约1014量级的SERS增强因子 聚集胶体银颗粒的近红外激发。 我们还演示了此增强功能不涉及 来自分子机制的贡献。正因为如此，方法 不仅适用于染料分子，也适用于无色 分子，从而将其适用范围扩展到生物分子。

The objective is to develop surface-enhanced Raman scattering (SERS) as an ultrasensitive analytical tool in chemistry, biology, and medicine. SERS effect combines sensitivity of the fluorescence spectroscopy with high structural information content of Raman spectroscopy, and can be used for single molecule detection and identification. Recently, we have achieved single molecule detection and identification of "non-absorbing"molecules by using specially prepared colloidal clusters and non resonant excitation. Extremely large SERS enhancement factors on the order of 1014 were obtained at near infra red excitation for aggregated colloidal silver particles. We have also demonstrated this enhancement does not involve contribution from molecular mechanisms. Because of this, the method is not only applicable to dye molecules but also to colorless molecules, thus extending the applicability to biological molecules.