RAMAN SPECTROSCOPIC EVIDENCE FOR Z DNA IN RAD ENZYME BOUND DNA

RAD 酶结合 DNA 中 Z DNA 的拉曼光谱证据

• 批准号: 6121254
• 负责人: IMRE BERGER
• 金额: $ 0.41万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121254
• 关键词: biological products; biomedical resource; genome; technology /technique

The objective is to develop surface-enhanced Raman scattering (SERS) as an ultrasensitive analytical tool in chemistry, biology, and medicine. SERS effect combines sensitivity of the fluorescence spectroscopy with high structural information content of Raman spectroscopy, and can be used for single molecule detection and identification. Recently, we have achieved single molecule detection and identification of "non-absorbing"molecules by using specially prepared colloidal clusters and non resonant excitation. Extremely large SERS enhancement factors on the order of 1014 were obtained at near infra red excitation for aggregated colloidal silver particles. We have also demonstrated this enhancement does not involve contribution from molecular mechanisms. Because of this, the method is not only applicable to dye molecules but also to colorless molecules, thus extending the applicability to biological molecules.

目的是发展表面增强拉曼散射 （Sers）作为化学，生物学和生物学中的超灵敏分析工具， 药 Sers效应结合了荧光的灵敏度 高结构信息含量拉曼光谱 光谱，并且可用于单分子检测， 识别. 最近，我们已经实现了单分子检测 并通过使用特殊的 制备胶体团簇和非共振激发。 极其 在1014的数量级上获得大的Sers增强因子， 聚集的胶态银颗粒的近红外激发。 我们还证明了这种增强并不涉及 分子机制的贡献。 因此，该方法 不仅适用于染料分子， 分子，从而将适用性扩展到生物分子。