RAMAN SPECTROSCOPIC EVIDENCE FOR Z DNA IN RAD ENZYME BOUND DNA

目标是开发表面增强拉曼散射 （SERS）作为化学、生物学和化学领域的超灵敏分析工具 药。 SERS 效应结合了荧光的灵敏度 具有高结构信息含量的拉曼光谱 光谱学，可用于单分子检测和 识别。 最近我们实现了单分子检测 并通过特殊方法识别“非吸收”分子 制备胶体簇和非共振激发。 极其 获得了 1014 量级的大 SERS 增强因子 聚集胶体银颗粒的近红外激发。 我们还证明了这种增强不涉及 分子机制的贡献。 正因为如此，该方法 不仅适用于染料分子，也适用于无色分子 分子，从而扩展了对生物分子的适用性。

The objective is to develop surface-enhanced Raman scattering (SERS) as an ultrasensitive analytical tool in chemistry, biology, and medicine. SERS effect combines sensitivity of the fluorescence spectroscopy with high structural information content of Raman spectroscopy, and can be used for single molecule detection and identification. Recently, we have achieved single molecule detection and identification of "non-absorbing"molecules by using specially prepared colloidal clusters and non resonant excitation. Extremely large SERS enhancement factors on the order of 1014 were obtained at near infra red excitation for aggregated colloidal silver particles. We have also demonstrated this enhancement does not involve contribution from molecular mechanisms. Because of this, the method is not only applicable to dye molecules but also to colorless molecules, thus extending the applicability to biological molecules.