RAMAN SPECTROSCOPIC EVIDENCE FOR Z DNA IN RAD ENZYME BOUND DNA

RAD 酶结合 DNA 中 Z DNA 的拉曼光谱证据

• 批准号: 6314168
• 负责人: IMRE BERGER
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314168
• 关键词: biological products; biomedical resource; nucleic acids

Existence of left handed DNA (Z-DNA) was discovered and extensively characterized in 1979. Since then there has been considerable research in establishing biological roles for Z-DNA. Recently Rich's group has established that a human protein, ds RNA deminase (dsRAD) has specificity for binding Z-DNA. The goal of this project is to provide direct evidence for the existence of left handed conformation for DNA when it binds to protein ds RAD. Raman spectra of poly(GC) in low salt and high salt solution, control protein, protein/poly (GC) complex have been obtained using 407 nm laser excitation. Raman spectral features of left and right handed DNA conformers have been characterized. By comparing these with the spectra of the protein/ poly (GC) complex, we concluded that DNA exists in left handed conformer when it binds to the ds RAD protein. Additional evidence have been provided by fluorescence and CD spectroscopy.

发现了左手DNA（Z-DNA）的存在， 1979年被广泛报道。 从那时起， 在确定Z-DNA的生物学作用方面进行了大量研究。 最近，里奇的研究小组已经确定，一种人类蛋白质，双链RNA， 脱氨酶（dsRAD）具有结合Z-DNA的特异性。 这个目标 该项目旨在为左撇子的存在提供直接证据 当它与蛋白质ds RAD结合时，DNA的构象。 拉曼光谱 聚（GC）在低盐和高盐溶液中的浓度，对照蛋白， 用407 nm激光制备了蛋白质/聚GC复合物 激发 左右手DNA的拉曼光谱特征 已表征了构象异构体。 通过将这些与 蛋白质/聚（GC）复合物的光谱，我们得出结论，DNA 当它与dsRAD蛋白结合时以左手构象体存在。 荧光和CD提供了额外的证据 谱