RAMAN SPECTROSCOPIC EVIDENCE FOR Z DNA IN RAD ENZYME BOUND DNA

发现了左手DNA(Z-DNA)的存在，并 在1979年被广泛地描述了。从那时起，一直有 在确定Z-DNA的生物学作用方面进行了大量研究。 最近，里奇的研究小组发现，一种名为ds RNA的人类蛋白质 脱氨酶(DsRAD)具有结合Z-DNA的特异性。这样做的目的是 该项目是为左撇子的存在提供直接证据 DNA与蛋白质DS RAD结合时的构象。拉曼光谱 低盐和高盐溶液中的聚(GC)，对照蛋白， 用407 nm激光获得了蛋白质/聚(GC)复合体 激动人心。左右手DNA的拉曼光谱特征 已经对构象进行了表征。通过将这些与 蛋白质/聚(GC)复合体的光谱，我们得出结论：DNA 当它与DS RAD蛋白结合时，存在于左手构象中。 荧光和CD提供了更多的证据 光谱学。

Existence of left handed DNA (Z-DNA) was discovered and extensively characterized in 1979. Since then there has been considerable research in establishing biological roles for Z-DNA. Recently Rich's group has established that a human protein, ds RNA deminase (dsRAD) has specificity for binding Z-DNA. The goal of this project is to provide direct evidence for the existence of left handed conformation for DNA when it binds to protein ds RAD. Raman spectra of poly(GC) in low salt and high salt solution, control protein, protein/poly (GC) complex have been obtained using 407 nm laser excitation. Raman spectral features of left and right handed DNA conformers have been characterized. By comparing these with the spectra of the protein/ poly (GC) complex, we concluded that DNA exists in left handed conformer when it binds to the ds RAD protein. Additional evidence have been provided by fluorescence and CD spectroscopy.