CENTER FOR CAENORHABDITIS ELEGANS ANATOMY

描述（改编自申请人的摘要）：C。elegans已经 被选为细胞发育的“高连通性”动物模型。 这种非常小的简单动物已被广泛用于实验 研究由于广泛的遗传资源和可访问的细胞生物学。 的 蠕虫基因组正在对蠕虫的完整基因组进行测序 财团 这种动物成年后的大部分解剖结构都是从 连续薄片的电子显微照片。 然而，EM技术 没有被C广泛使用。elegans社区，和以前的大多数数据 EM研究不容易获得。 梭elegans解剖中心将 集中精力扩展这些EM方法在C.尤其是对 研究正常发育和突变动物中的细胞变化， 进行EM免疫细胞化学研究，以定位抗原在 超微结构水平 这些研究将在密切 与NIH和NSF资助的实验室合作，作为指导性组成部分 他们正在进行的研究工作。 此外，中心还将发布 更详细的信息，关于解剖的线虫在 注释电子显微照片的形式。 这些信息将提供给 在CD-ROM上，在万维网上，并且可能以书的形式。 哪里 必要时，中心将对野生型进行额外的EM调查 解剖在几个发展阶段，以补充地图集， 尤其是填补一般知识的空白。 此前资源 应用程序将建立该中心的能力超过最初的三个 年期间。 申请人打算申请P40或P41补助金， 继续支持中心。

DESCRIPTION (Adapted from the applicant's abstract): C. elegans has been selected as a "high connectivity" animal model for cellular development. This very small, simple animal has been widely adopted for experimental study due to extensive genetic resources and accessible cell biology. The complete genome of the worm is being sequenced by the Worm Genome Consortium. Much of the animal's adult anatomy has been reconstructed from electron micrographs of serial thin sections. However, EM techniques are not widely utilized by the C. elegans community, and most data from previous EM studies are not easily accessed. The C. elegans Anatomy Center will concentrate on extending these EM methods in C. elegans, particularly to study cellular changes during normal development and in mutant animals, and to conduct EM-immunocytochemical studies to localize antigens at the ultrastructural level. Such studies will be conducted in close collaboration with NIH- and NSF-funded laboratories, as a directed component of their ongoing research efforts. In addition, the Center will publish much more detailed information regarding the anatomy of the nematode in the form of annotated electron micrographs. This information will be supplied on CD-ROMs, over the World Wide Web, and possibly in book form. Where necessary, the Center will conduct additional EM surveys of the wild type anatomy at several developmental stages to supplement the Atlas, particularly to fill gaps in general knowledge. This pre-resource application will build the capabilities of the Center over an initial three year period. The applicants intend to request a P40 or P41 grant later for continued support of the Center.