GENETIC MANAGEMENT OF CHIMPANZEE BREEDING AND RESEARCH

黑猩猩育种和研究的遗传管理

• 批准号: 6033708
• 负责人: John Ely
• 金额: $ 17.06万
• 依托单位: BIOQUAL, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-01-15 至 2001-03-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6033708
• 关键词: Pan; animal breeding; animal colony; animal population genetics; cooperative study; genetic markers; genetic polymorphism; hepatitis C; immunogenetics; information systems; nucleic acid repetitive sequence

The objective in establishing the Chimpanzee Breeding and Research Program (CBRP) breeding colonies was to insure a steady supply of healthy chimpazees for future biomedical research needs. Genetic management of these chimpanzee colonies is a prerequisite for this goal. Sound genetic management requires genetic markers, which are essential for accurate pedigrees as well as for many research protocols. Thus, genetic management must begin with a search for informative new DNA markers, utilize them in paternity testing and the generation of high-quality corroborated pedigree data, yet still take probable future research needs into account. Since genetic management cannot effectively proceed without an explicit research effort, genetic management activities will be integrated with quantitative genetic analyses designed to exploit pre- existing research resources. Toward these ends, human short tandem repeat (STR) DNA markers which can be typed using PCR will be identified which reveal informative polymorphisms in chimpanzees. These markers will be used for paternity testing and pedigree corroboration, and for large- scale DNA typing of wild-born CBRP chimpanzees. These data will be used to characterize the amount of genetic variability present in African-born founders of the CBRP breeding populations, evaluate the degree of genetic divergence among the five CBRP colonies, formulate colony-specific breeding advice and identity replacement breeders. Pedigree data corroborated with DNA markers will also be analyzed with the tools of quantitative genetics to study the genetic basis of viral disease. Collaborative research will focus on the genetic epidemiology of Hepatitis C, the most frequent cause of transfusion-associated hepatitis in the United States. Over 240 chimpanzees have already been infected with HCV as part of earlier immunovirological research related to vaccine development. We will utilize these pre-existing data in a collaboration involving DNA typing, quantitative genetics, and immunophenotyping to investigate the genetic epidemiology of this infectious disease and thereby fully exploit the research value of these essential animal models.

建立黑猩猩育种和研究中心的目标 CBRP 育种群计划旨在确保健康的稳定供应 黑猩猩以满足未来生物医学研究的需要。基因管理 这些黑猩猩群体是实现这一目标的先决条件。 健全遗传 管理需要遗传标记，这对于准确的管理至关重要 谱系以及许多研究方案。因此，遗传 管理层必须从寻找信息丰富的新 DNA 标记开始， 将它们用于亲子鉴定和生成高质量的 证实的谱系数据，但仍需要未来可能的研究需求 考虑到。由于如果没有基因管理就无法有效进行 明确的研究工作，遗传管理活动将 与旨在利用预分析的定量遗传分析相结合 现有的研究资源。为了实现这些目标，人类短串联重复序列 (STR) 可使用 PCR 分型的 DNA 标记将被识别 揭示黑猩猩的信息多态性。这些标记将 用于亲子鉴定和谱系证实，以及大型 野生出生的 CBRP 黑猩猩的规模 DNA 分型。这些数据将被使用 描述非洲出生的人中存在的遗传变异量 CBRP育种群体的创始人，评估遗传程度 五个 CBRP 菌落之间的差异，制定菌落特异性 育种建议和身份更换育种者。谱系数据 DNA标记证实也将使用以下工具进行分析 定量遗传学研究病毒性疾病的遗传基础。 合作研究将集中于遗传流行病学 丙型肝炎是输血相关性肝炎的最常见原因 在美国。超过240只黑猩猩已被感染 HCV 作为与疫苗相关的早期免疫病毒学研究的一部分 发展。我们将在合作中利用这些预先存在的数据 涉及 DNA 分型、定量遗传学和免疫表型分析 调查该传染病的遗传流行病学并 从而充分发挥这些重要动物模型的研究价值。