HORMONAL MANIPULATION OF MENSTRUAL CYCLES IN MACAQUES

这是对其他调查人员服务的延续 提供已知孕周的怀孕，并提供 恒河猴(猕猴)的同步月经周期。为 定时繁殖，猴子？循环由每日控制 注射黄体酮(5 mg/d)，连续10天。雌性是配对的 在最后一次注射黄体酮后第14至21天与雄性交配。 排卵和受孕发生在黄体酮分泌后的第16至18天 打针。成功率，以怀孕21天的存活胎儿为基础， 对于第一周期的匹配，保持在60%到70%。胎儿发育是 通过超声检查进行监测。用于月经同步 周期猴每天接受两次检查，以检测月经。在16号 每天注射黄体酮的周期开始了。注射是 继续，直到所有要同步的猴子都收到 至少注射10次。在这一点上，所有注射都停止了。月经 发生在最后一次黄体酮注射后的第一天或第二天。如果 研究人员希望治疗处于黄体期的猴子 周期，黄体酮注射可以在第16-18天再次开始， 为了这项研究，猴子可以保持在黄体期。 资助基础赠款出版物无

This is a continuation of services to other investigators to provide pregnancies of known gestational ages and to provide synchronized menstrual cycles in rhesus monkeys (Macaca mulatta). For timed breeding, the monkeys? cycles are controlled by the daily injection of progesterone (5 mg/day) for 10 days. Females are paired with males on days 14 to 21 after the last progesterone injection. Ovulation and conception occurs on day 16 to 18 after the progesterone injections. Success rate, based on viable fetus at 21 days gestation, remains at 60% to 70% for first cycle matings. Fetal development is monitored using ultrasonography. For synchronization of menstrual cycles monkeys are examined twice daily to detect menses. On the 16th days of the cycle daily progesterone injections begin. Injections are continued until all the monkeys to be synchronized have received a minimum of 10 injections. At that point all injections cease. Menses occurs on day 1 or 2 following the last progesterone inj ection. If the investigator wishes to treat the monkeys in the luteal stage of the cycle, progesterone injections can begin again on day 16 - 18 and the monkeys can be maintained in a luteal stage for the study. FUNDING Base Grant PUBLICATIONS None