ENDOCRINOLOGY CORE LABORATORY

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。在过去的一年里，ART核心在TNPRC进行了辅助生殖技术(ART)的区域影响评估以及其他几项PI。所有使用的RIA试剂盒均购自诊断产品公司。这些试剂盒包括标准品、I125标记的激素和与特定抗体结合的试管。该试验是一种竞争性结合试验。结果是根据与试管结合的标记荷尔蒙的量来确定的，并与标准进行比较。TNPRC开发的计算机程序被用来建立最佳拟合曲线，结果在分析后6至48小时内提供给调查人员，具体取决于所分析的样本数量。检测的次数包括测定血清雌二醇(1626次)、皮质醇(1338次)和孕酮(394次)的浓度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the past year the ART Core has conducted RIAs for the Assisted Reproductive Technology (ART) as well as several other PIs at the TNPRC. All RIA assays utilized kits were purchased from Diagnostic Products Corporation. These kits include standards, I125 labeled hormone, and tubes bound with specific antibody. The assay is a competitive binding assay. Results were determined based on the amount of labeled hormone bound to the tubes and compared to the standards. A computer program developed at the TNPRC was used to establish as ¿best fit¿ curve and results were provided to the investigators within 6 to 48 hours of the assay, depending on the number of samples assayed. The number of assays included determination of serum concentration for estradiol (1626 assays) cortisol (1338 assays) ) and progesterone (394 assays).