SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL

[1,3_2 H4, 2 13C]甘油的合成，

• 批准号: 6120866
• 负责人: RODOLFO MARTINEZ
• 金额: $ 2.74万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6120866
• 关键词: biological products; biomedical resource; enzymes; nucleic acids

David LeMaster has developed mutants of E. coli that allow for labeling of proteins in alternate carbons. These organism are cultured on glycerol labeled either at C2 or C I and C3. Proteins expressed in this background are labeled at alternate carbons and are used to stud side chains motion by NMR relaxation methods. The alternate labeling is key to the success of this approach, because the complications that arise from 13C_ "C dipolar interactions present in uniformly labeled proteins have been eliminated. SIR has incorporated deuterium specifically into 1,3- 13 C2- and 2-"C-labeled glycerol which will result in more isolated 13C_ 'H vectors for which dynamics will be interpretable.

David LeMaster 开发了大肠杆菌突变体，可以 在交替碳中标记蛋白质。 这些生物体是 在标记为 C2 或 C I 和 C3 的甘油上培养。 蛋白质 在这个背景中表达的在替代碳上被标记并且是 用于通过 NMR 弛豫方法研究侧链运动。 这 替代标签是这种方法成功的关键，因为 13C_“C 偶极相互作用引起的并发症 统一标记的蛋白质已被消除。 SIR 已合并 氘特异性地转化为 1,3- 13 C2- 和 2-"C- 标记的甘油 这将导致更多孤立的 13C_'H 向量，其动力学 将是可解释的。