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SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL

SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL
[1,3_2 H4, 2 13C]甘油的合成，
批准号：
6281485
负责人：
RODOLFO MARTINEZ
金额：
$6.21万
依托单位：
UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-01-15 至 1999-01-14
来源：
https://reporter.nih.gov/project-details/6281485
关键词：
biological products biomedical resource enzymes nucleic acids

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中文摘要
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英文摘要
David LeMaster has developed mutants of E. coli that allow for labeling of proteins in alternate carbons. These organism are cultured on glycerol labeled either at C2 or C I and C3. Proteins expressed in this background are labeled at alternate carbons and are used to stud side chains motion by NMR relaxation methods. The alternate labeling is key to the success of this approach, because the complications that arise from 13C_ "C dipolar interactions present in uniformly labeled proteins have been eliminated. SIR has incorporated deuterium specifically into 1,3- 13 C2- and 2-"C-labeled glycerol which will result in more isolated 13C_ 'H vectors for which dynamics will be interpretable.
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SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL
AMINO ACID LABELING FOR LARGER PROTEINS METHYL GROUP LABELING
SYNTHESIS OF LABELED PRECURSORS
AMINO ACID LABELING FOR LARGER PROTEINS METHYL GROUP LABELING
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