课题基金 / 基金详情

SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL

SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL
[1,3_2 H4, 2 13C]甘油的合成，
批准号：
6309027
负责人：
RODOLFO MARTINEZ
金额：
$2.74万
依托单位：
UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
项目状态：
已结题
起止时间：
2000-02-01 至 2002-01-14
来源：
https://reporter.nih.gov/project-details/6309027
关键词：
biological products biomedical resource enzymes nucleic acids

项目摘要

项目成果

RODOLFO MARTINEZ的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
David LeMaster has developed mutants of E. coli that allow for labeling of proteins in alternate carbons. These organism are cultured on glycerol labeled either at C2 or C I and C3. Proteins expressed in this background are labeled at alternate carbons and are used to stud side chains motion by NMR relaxation methods. The alternate labeling is key to the success of this approach, because the complications that arise from 13C_ "C dipolar interactions present in uniformly labeled proteins have been eliminated. SIR has incorporated deuterium specifically into 1,3- 13 C2- and 2-"C-labeled glycerol which will result in more isolated 13C_ 'H vectors for which dynamics will be interpretable.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
AMINO ACID LABELING FOR LARGER PROTEINS METHYL GROUP LABELING
SYNTHESIS OF LABELED PRECURSORS
AMINO ACID LABELING FOR LARGER PROTEINS METHYL GROUP LABELING
SYNTHESIS OF LABELED PRECURSORS
海外基金