SYNTHESIS OF [1,3_2 H4, 2 13C] GLYCEROL, & [1,2,3_2 H31 1,3 13 C21GLYCEROL

[1,3_2 H4, 2 13C]甘油的合成，

• 批准号: 6309027
• 负责人: RODOLFO MARTINEZ
• 金额: $ 2.74万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-02-01 至 2002-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6309027
• 关键词: biological products; biomedical resource; enzymes; nucleic acids

David LeMaster has developed mutants of E. coli that allow for labeling of proteins in alternate carbons. These organism are cultured on glycerol labeled either at C2 or C I and C3. Proteins expressed in this background are labeled at alternate carbons and are used to stud side chains motion by NMR relaxation methods. The alternate labeling is key to the success of this approach, because the complications that arise from 13C_ "C dipolar interactions present in uniformly labeled proteins have been eliminated. SIR has incorporated deuterium specifically into 1,3- 13 C2- and 2-"C-labeled glycerol which will result in more isolated 13C_ 'H vectors for which dynamics will be interpretable.

大卫·勒马斯特已经培育出了E.大肠杆菌， 在交替碳中标记蛋白质。 这些生物体是 在C2或C1和C3标记的甘油上培养。 蛋白 在该背景中表达的是在交替的碳原子处标记的， 用于NMR弛豫方法研究侧链运动。 的 交替标记是这种方法成功的关键，因为 13 C_“C偶极相互作用引起的复杂性， 已经消除了均匀标记的蛋白质。 SIR已纳入 氘特异性地进入1，3 - 13 C2-和2-“C-标记的甘油 这将导致更多孤立的13 C_1H矢量， 将被解读。