Development of an immersion vaccine for salmonids

鲑鱼浸泡疫苗的开发

• 批准号: NE/P010873/1
• 负责人: Pieter Van West
• 金额: $ 25.78万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2017
• 资助国家: 英国
• 起止时间: 2017 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FP010873%2F1
• 关键词: Development; immersion; vaccine; salmonids

Fish handling and injection-based vaccination is one of the biggest causes of stress for fish and always results in increased mortality due to post-vaccination outbreaks of saprolegniosis and bacterial infections. In Scotland alone, millions of pre-smolt (10% of all hatched salmon) die due to Saprolegnia infections after receiving an injection-based vaccine. Therefore, there is an urgent need to develop better vaccination practices to increase animal welfare and reduce mortalities. Immersion vaccination of fish was the first method used to demonstrate effective protection against bacterial diseases, but was overtaken by injection vaccination due to the need for inclusion of vaccine adjuvants. Should enhanced delivery of antigens be possible by the immersion route clearly this holds enormous promise to completely obviate fish handling, thus reducing stress and significantly lowering mortality. The central hypothesis of our programme is that immersion vaccination with recombinant SpHtp1-coupled antigens can be used successfully to protect fish against diseases. Our overall objective is to develop and commercialise the SpHtp1 translocation mechanism into an immersion vaccine delivery method for salmonids and other farmed fish. We have already demonstrated that our patented method can be used successfully and effectively in directing proteins into live fish. Furthermore, we have observed specific antibody production in trout that were immersed in a solution of SpHtp1 fused to mRFP.Therefore, our main outputs will be: 1) Evidence that the translocating peptide of SpHtp1 coupled to selected antigens give protection against disease of concern in salmonid aquaculture, in immersion vaccination trial experiments.2) Optimisation of the immune response in fish.3) A highly efficient and productive SpHtp1-recombinant protein overexpression method for large-scale production and future commercialisation of the vaccine method by our commercial partners BAHL and Pulcea Ltd.4) A method to recycle and inactivate the immersion vaccine that can be employed on site in the farm.All outputs will be exploited by BAHL and Pulcea Ltd. in obtaining licenses and approvals for commercialisation and eventually full use by the aquaculture industry. Antigens of essential pathogens are already available through BAHL, and further antigens will be sought in future projects by the UoA and BAHL. The current application is ambitious but achievable and will take 24 months to complete because of the significant amount of work that is proposed. In summary, the potential impact of an immersion vaccine for finfish is "aquaculture industry-transformational" as fish vaccinated by injection could be eliminated, which would greatly increase the welfare of the fish and minimise fish losses and thus financial losses in the aquaculture business worldwide.

鱼类处理和注射疫苗接种是造成鱼类应激的最大原因之一，并且由于疫苗接种后爆发水霉病和细菌感染，总是会导致死亡率增加。仅在苏格兰，数百万幼龄小鲑鱼（占所有孵化鲑鱼的 10%）在接受注射疫苗后因水霉属感染而死亡。因此，迫切需要开发更好的疫苗接种实践，以提高动物福利并降低死亡率。鱼的浸泡接种是第一种被证明可以有效预防细菌性疾病的方法，但由于需要包含疫苗佐剂而被注射接种所取代。如果可以通过浸泡途径增强抗原的递送，显然这对于​​完全避免鱼的处理具有巨大的希望，从而减少压力并显着降低死亡率。我们项目的中心假设是，重组 SpHtp1 偶联抗原的浸没式疫苗接种可以成功地用于保护鱼类免受疾病侵害。我们的总体目标是将 SpHtp1 易位机制开发并商业化为鲑鱼和其他养殖鱼类的浸入式疫苗递送方法。我们已经证明，我们的专利方法可以成功有效地将蛋白质导入活鱼中。此外，我们还观察到，将鳟鱼浸入与 mRFP 融合的 SpHtp1 溶液中，鳟鱼会产生特异性抗体。因此，我们的主要成果将是：1) 证据表明，在浸没疫苗接种试验中，SpHtp1 的易位肽与选定的抗原偶联，可以预防鲑科鱼水产养殖中关注的疾病。2) 鱼类免疫反应的优化。3) 高度 高效、多产的 SpHtp1 重组蛋白过表达方法，用于大规模生产和未来商业化，由我们的商业合作伙伴 BAHL 和 Pulcea Ltd. 4) 一种回收和灭活浸没疫苗的方法，可在农场现场使用。BAHL 和 Pulcea Ltd. 将利用所有产出获得商业化的许可和批准，并最终 水产养殖业充分利用。 BAHL 已经提供了基本病原体的抗原，并且 UoA 和 BAHL 将在未来的项目中寻找更多抗原。目前的申请雄心勃勃但可以实现，并且由于拟议的工作量很大，需要 24 个月才能完成。总之，有鳍鱼类浸泡疫苗的潜在影响是“水产养殖业的转型”，因为注射疫苗的鱼类可以被淘汰，这将大大增加鱼类的福利，并最大限度地减少鱼类损失，从而减少全球水产养殖业的经济损失。

项目成果

Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany.

• DOI: 10.3390/jof7110968
• 发表时间: 2021-11-13
• 期刊: Journal of fungi (Basel, Switzerland)
• 作者: Masigol H;Woodhouse JN;van West P;Mostowfizadeh-Ghalamfarsa R;Rojas-Jimenez K;Goldhammer T;Khodaparast SA;Grossart HP
• 通讯作者: Grossart HP

Transcriptome analysis reveals immune pathways underlying resistance in the common carp Cyprinus carpio against the oomycete Aphanomyces invadans.

• DOI: 10.1016/j.ygeno.2020.10.028
• 发表时间: 2020-10
• 期刊: Genomics
• 影响因子: 4.4
• 作者: D. K. Verma;L. Peruzza;L. Peruzza;F. Trusch;F. Trusch;M. Yadav;Ravindra;Sergei V. Shubin;K. Mo

Cell entry of a host-targeting protein of oomycetes requires gp96.

• DOI: 10.1038/s41467-018-04796-3
• 发表时间: 2018-06-14
• 期刊: Nature communications
• 影响因子: 16.6
• 作者: Trusch F;Loebach L;Wawra S;Durward E;Wuensch A;Iberahim NA;de Bruijn I;MacKenzie K;Willems A;Toloczko A;Diéguez-Uribeondo J;Rasmussen T;Schrader T;Bayer P;Secombes CJ;van West P
• 通讯作者: van West P

The chaperone Lhs1 contributes to the virulence of the fish-pathogenic oomycete Aphanomyces invadans.

伴侣 Lhs1 有助于鱼类致病性卵菌 Aphanomyces invadans 的毒力。

• DOI: 10.1016/j.funbio.2020.09.003
• 发表时间: 2020
• 期刊: Fungal biology
• 影响因子: 2.5
• 作者: Iberahim NA