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Biochemical characterisation of the translocation process of RxLR-like effector proteins via tyrosine-O-sulphate modified cell surface receptors

RxLR样效应蛋白通过酪氨酸-O-硫酸盐修饰的细胞表面受体易位过程的生化表征

基本信息

批准号：
BB/J018333/1
负责人：
Pieter Van West
金额：
$ 46.01万
依托单位：
University of Aberdeen
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2013
资助国家：
英国
起止时间：
2013 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FJ018333%2F1
关键词：
Biochemical characterisation translocation process RxLR

项目摘要

The United Nations predict that by 2050 about 9-10 billion people will populate the world. To provide enough food for the growing world population requires that areas of food production that still have the capacity to grow, like aquaculture, will be exploited more rigorously in the future but also that losses due to diseases need to be reduced. Our aim is to understand early host pathogen interactions of oomycete parasites that heavily impact food production. Oomycetes are fungal-like organisms that cause diseases on plants and animals. For example different Saprolegnia species infects fish (Saprolegniosis) at different development levels. In order to cause disease these parasites need to deliver molecules into their host cells to manipulate the host in such a way that it can infect and propagate successfully. One group of such molecules are the so called effectors. These molecules do not only target specific host molecules but some also seem to highjack host transport mechanisms in order to enter the cells. We will characterise the molecular mechanisms that effector proteins use to translocate into host cells, since a detailed understanding of these processes will help us to develop methods/substances that can block the infection process. This will allow fish to fight off their oomycete attackers more efficiently. In addition, we would like to use the 'effector entering system' to develop an immersion vaccine whereby fish do not need to be handled and injected anymore. Such a vaccine would reduce stress in fish and the occurrence of vaccination associated diseases, which includes Saprolegniosis.

联合国预测，到2050年，世界人口将达到90 - 100亿。为了向不断增长的世界人口提供足够的粮食，需要在未来更严格地开发仍有增长能力的粮食生产领域，如水产养殖，但也需要减少疾病造成的损失。我们的目的是了解卵菌寄生虫的早期宿主病原体相互作用，严重影响粮食生产。卵菌是一种真菌样的生物体，能引起动植物疾病。例如，不同的水霉属物种在不同的发育水平感染鱼类（水霉病）。为了引起疾病，这些寄生虫需要将分子输送到宿主细胞中，以操纵宿主，使其能够成功感染和繁殖。一类这样的分子是所谓的效应物。这些分子不仅靶向特定的宿主分子，而且一些分子似乎还劫持宿主转运机制以进入细胞。我们将研究效应蛋白用于易位到宿主细胞中的分子机制，因为对这些过程的详细了解将有助于我们开发可以阻断感染过程的方法/物质。这将使鱼类更有效地击退卵菌攻击者。此外，我们希望使用“效应器进入系统”来开发一种浸入式疫苗，从而不再需要处理和注射鱼类。这种疫苗将减少鱼类的压力和疫苗接种相关疾病的发生，包括水霉病。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Parental transfer of the antimicrobial protein LBP/BPI protects Biomphalaria glabrata eggs against oomycete infections.

DOI：
10.1371/journal.ppat.1003792
发表时间：
2013
期刊：
PLoS pathogens
影响因子：
6.7
作者：
Baron OL;van West P;Industri B;Ponchet M;Dubreuil G;Gourbal B;Reichhart JM;Coustau C
通讯作者：
Coustau C

NmPin from the marine thaumarchaeote Nitrosopumilus maritimus is an active membrane associated prolyl isomerase.

DOI：
10.1186/s12915-016-0274-1
发表时间：
2016-06-27
期刊：
BMC biology
影响因子：
5.4
作者：
Hoppstock L;Trusch F;Lederer C;van West P;Koenneke M;Bayer P
通讯作者：
Bayer P

Export of malaria proteins requires co-translational processing of the PEXEL motif independent of phosphatidylinositol-3-phosphate binding.

DOI：
10.1038/ncomms10470
发表时间：
2016-02-01
期刊：
Nature communications
影响因子：
16.6
作者：
Boddey JA;O'Neill MT;Lopaticki S;Carvalho TG;Hodder AN;Nebl T;Wawra S;van West P;Ebrahimzadeh Z;Richard D;Flemming S;Spielmann T;Przyborski J;Babon JJ;Cowman AF
通讯作者：
Cowman AF

New record and phylogenetic affinities of the oomycete Olpidiopsis feldmanni infecting Asparagopsis sp. (Rhodophyta)

感染 Asparagopsis sp 的卵菌 Olpidiopsis feldmanni 的新记录和系统发育亲和力。