CORE--VIROLOGY

核心--病毒学

• 批准号: 6099810
• 负责人: F BLAINE HOLLINGER
• 金额: $ 11.41万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099810
• 关键词: AIDS /HIV diagnosis; HIV infections; biomedical facility; human immunodeficiency virus 1; human subject; lymphocyte; plasma; polymerase chain reaction; serology /serodiagnosis; serum; virus antigen

The creation of a Virology Core for the CFAR will provide investigators with a state-of-the-art facility that will have the capability and capacity to perform all aspects of HIV virology testing. The overall goals of the Virology Core will be to provide laboratory assistance to investigators requiring sophisticated protocols for assessing virologic endpoints to therapy, to provide expert assistance for the interpretation of results to clinicians involved in the care of patients with HIV infection, and to provide instruction and technical advice to researchers who are interested in developing sophisticated virologic procedures for their HIV projects. Specifically, the Virology Core will: (1) receive, process, and test plasma or serum, PBMC, tissues, secretions and excretions from patients with HIV; (2) isolated and propagate HIV from these samples; (3) perform HIV-specific diagnostic assays for measurement of viral or surrogate markers using biochemical, immunologic, and molecular approaches; (4) perform gene ampliation techniques to assist in the detection of HIV-specific RNA or proviral DNA from clinical specimens using polymerase chain reaction (PCR) methodology; (5) evaluate drug susceptibility of HIV isolates; and (6) serve as a repository for the long-term storage of clinical specimens from adults infected with HIV and maintain a computerized freezer storage and tracking program so that such specimens will be available for subsequent research programs.

为CFAR创建病毒学核心将为研究人员提供 拥有最先进的设施， 进行艾滋病毒检测的所有方面的能力。 整体 病毒学核心的目标是提供实验室援助， 研究者需要复杂的方案来评估病毒学 治疗终点，为解释提供专家帮助 向参与艾滋病患者护理的临床医生提供结果 感染，并为研究人员提供指导和技术咨询 他们有兴趣开发复杂的病毒学程序， 艾滋病项目。 具体而言，病毒学核心将：（1）接收， 处理和检测血浆或血清、PBMC、组织、分泌物和 HIV感染者的排泄物;（2）分离和传播HIV， 这些样品;（3）进行HIV特异性诊断测定， 病毒或替代标志物的生物化学，免疫学， 分子方法;（4）进行基因扩增技术，以帮助 在检测HIV特异性RNA或前病毒DNA中， 采用聚合酶链反应（PCR）方法检测标本;（5）评价 HIV分离株的药物敏感性;（6）作为储存库， 长期储存感染艾滋病毒的成人临床标本 并保持一个计算机化的冷冻存储和跟踪程序， 这些标本将可用于随后的研究计划。