HEPATITIS C VIRUS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 HCV是一种血液传播的病原体，感染约3%的人口。 超过70%的病毒感染者最终会患上慢性肝炎，这可能导致肝硬化、肝细胞癌、自身免疫性疾病和近30种其他相关疾病。最初，HCV感染是无症状的。感染20-30年后，大多数患者会出现慢性肝脏感染的症状（疲劳和食欲不振），并伴有AST和ALT等肝酶水平升高 1)使用在肝损伤或特异性抗HCV抗体发生之前的感染早期阶段收集的感染血液，通过冷冻电镜确定丙型肝炎病毒（HCV）及其分子组分的三维结构 2)分析包膜蛋白E1 E2的分子结构，并建立与细胞受体相互作用的模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HCV is a blood borne pathogen that infects about 3% of the human population. More than 70% of people infected with the virus eventually get chronic hepatitis which can cause cirrhosis of the liver, hepatocellular carcinoma, autoimmune diseases, and almost 30 other associations. Initially, HCV infection is asymptomatic. After 20-30 years of infection, most patients will have symptoms (fatigue and poor appetite) of chronic liver infection with elevated levels of liver enzymes such as AST and ALT 1) Determine the 3D structure of hepatitis C virus (HCV) and its molecular components by cryo-EM using infectious blood collected during the early stages of infection prior to the development of liver injury or specific anti-HCV antibody 2) Analyze the molecular structure of the envelope proteins, E1E2, and develop a model of interaction with cellular receptors.