HEPATITIS C VIRUS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。丙型肝炎病毒是一种血源性病原体，约有3%的人感染。超过70%的感染者最终患上慢性肝炎，慢性肝炎可导致肝硬化、肝细胞癌、自身免疫性疾病和近30种其他相关疾病。最初，HCV感染是无症状的。感染20-30年后，大多数患者出现慢性肝脏感染的症状（疲劳、食欲不振），肝酶如AST、ALT水平升高1)利用感染早期肝损伤前或特异性抗HCV抗体采集的感染性血液，用冷冻电镜（cro - em）测定丙型肝炎病毒（HCV）的三维结构及其分子成分2)分析包膜蛋白E1E2、建立一个与细胞受体相互作用的模型。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HCV is a blood born pathogen that infects about 3% of the human population. More than 70% of people infected with the virus eventually get chronic hepatitis which can cause cirrhosis of the liver, hepatocellular carcinoma, autoimmune diseases, and almost 30 other associations. Initially, HCV infection is asymptomatic. After 20-30 years of infection, most of patients will have symptoms (fatigue and poor appetite) of chronic liver infection with elevated levels of liver enzymes such as AST and ALT 1) Determine the 3D structure of hepatitis C virus (HCV) and its molecular components by cryo-EM using infectious blood collected during the early stages of infection prior to the development of liver injury or specific anti-HCV antibody 2) Analyze the molecular structure of the envelope proteins, E1E2, and develop a model of interaction with cellular receptors.