A YEAST ARTIFICIAL CHROMOSOME CONTIG MAP OF CHROMOSOME 12
A YEAST ARTIFICIAL CHROMOSOME CONTIG MAP OF CHROMOSOME 12
批准号:
6109106
负责人:
Raju S. Kucherlapati
金额:
$12.24万
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-18 至 2000-06-30
关键词:
DNA artificial chromosomes chromosomes computer assisted sequence analysis cytogenetics fluorescent dye /probe fungal genetics genetic library genetic mapping genetic markers genome human genetic material tag human tissue in situ hybridization molecular cloning nucleic acid probes nucleic acid sequence polymerase chain reaction pulsed field gel electrophoresis sequence tagged sites
中文摘要
项目2的目标是开发一个高分辨率的序列标记
英文摘要
The goal of Project 2 is to develop a high resolution sequence tagged
site (STS) content based map of chromosome 12. We propose a program to
build this map in close association with Dr. Daniel Cohen and his
colleagues at CEPH in Paris who are developing a low resolution physical
map for the entire human genome. We will obtain a chromosome 12 specific
sublibrary of the large insert yeast artificial chromosome (YAC)
libraries recently constructed in Paris. In addition, Dr. Cohen will
provide us with information about those YACs in the chromosome 12
sublibrary which have been assembled into contigs by the fingerprinting
methods they employ. We will ascertain the size of the individual YACs
in these contigs generate end-specific sequences from each of the YACs.
All end sequences which are determined to be derived from chromosome 12
will be sequenced, at least in part, to generate STSs. Each of the YACs
within the contig will be screened for the presence or absence of each
STSs. The binary information obtained will be used to construct an STS-
content based map. We propose to utilize homologous recombination
methods to construct an extremely high resolution map in which the
precise order and distance between STSs will be deduced. We propose a
combination of methods to identify gaps and to fill them to complete the
physical map of chromosome 12. In addition to the YAC-end specific STS,
we will use all of the genetic markers that have been utilized and that
are being developed by several groups in constructing the chromosome 12
physical map. This procedure enables us to integrate the genetic maps
being constructed elsewhere with the physical maps we will construct.
The genetic distances between pairs of markers can be compared with
precise physical distances that we will obtain to identify regions of
high or low recombination. To fill in the gaps in the genetic map, we
propose a directed effort to isolate microsatellite-containing sequences
from specific YACs and use them to type the CEPH pedigrees. Our physical
mapping effort will complement and build upon the global efforts at CEPH
and other institutions and could provide a new paradigm for constructing
high resolution maps of individual human chromosomes.
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Harvard Genome Characterization Center
-
批准号:7942761
-
项目类别:
-
资助金额:$218.6万
-
财政年份:2009
-
负责人:Raju S. Kucherlapati
-
依托单位:
Harvard Genome Characterization Center
-
批准号:9198334
-
项目类别:
-
资助金额:$16.85万
-
财政年份:2009
-
负责人:Raju S. Kucherlapati
-
依托单位:
Harvard Genome Characterization Center
-
批准号:8528373
-
项目类别:
-
资助金额:$253.9万
-
财政年份:2009
-
负责人:Raju S. Kucherlapati
-
依托单位:
Harvard Genome Characterization Center
-
批准号:8322121
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项目类别:
-
资助金额:$200.91万
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财政年份:2009
-
负责人:Raju S. Kucherlapati
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依托单位:
Cancer Genomics Center
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批准号:7908618
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项目类别:
-
资助金额:$64.93万
-
财政年份:2009
-
负责人:Raju S. Kucherlapati
-
依托单位:
Harvard Genome Characterization Center
-
批准号:7788991
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项目类别:
-
资助金额:$300.0万
-
财政年份:2009
-
负责人:Raju S. Kucherlapati
-
依托单位:
Harvard Genome Characterization Center
-
批准号:8124973
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项目类别:
-
资助金额:$206.12万
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财政年份:2009
-
负责人:Raju S. Kucherlapati
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依托单位:
Career Development Program
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批准号:9112897
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项目类别:
-
资助金额:$10.38万
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财政年份:2007
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负责人:Raju S. Kucherlapati
-
依托单位:
Career Development Program
-
批准号:8933248
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项目类别:
-
资助金额:$10.03万
-
财政年份:2007
-
负责人:Raju S. Kucherlapati
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依托单位:
Molecular Flourescent Imaging for the Early Detection of Colorectal Neoplasia
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批准号:7248206
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项目类别:
-
资助金额:$33.42万
-
财政年份:2007
-
负责人:Raju S. Kucherlapati
-
依托单位:
Career Development Program
-
批准号:8485731
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项目类别:
-
资助金额:$7.39万
-
财政年份:2007
-
负责人:Raju S. Kucherlapati
-
依托单位:
Cancer Genomics Center
-
批准号:7294859
-
项目类别:
-
资助金额:$188.23万
-
财政年份:2006
-
负责人:Raju S. Kucherlapati
-
依托单位:
Cancer Genomics Center
-
批准号:7233887
-
项目类别:
-
资助金额:$170.85万
-
财政年份:2006
-
负责人:Raju S. Kucherlapati
-
依托单位:
Cancer Genomics Center
-
批准号:7499586
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项目类别:
-
资助金额:$185.52万
-
财政年份:2006
-
负责人:Raju S. Kucherlapati
-
依托单位:
Comparative Genetics of the DiGeorge Syndrome Gene TBX1
-
批准号:6999354
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项目类别:
-
资助金额:$30.98万
-
财政年份:2005
-
负责人:Raju S. Kucherlapati
-
依托单位:
Comparative Genetics of the DiGeorge Syndrome Gene TBX1
-
批准号:7544538
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项目类别:
-
资助金额:$29.48万
-
财政年份:2005
-
负责人:Raju S. Kucherlapati
-
依托单位:
Comparative Genetics of the DiGeorge Syndrome Gene TBX1
-
批准号:7332242
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项目类别:
-
资助金额:$29.48万
-
财政年份:2005
-
负责人:Raju S. Kucherlapati
-
依托单位:
Comparative Genetics of the DiGeorge Syndrome Gene TBX1
-
批准号:7156974
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项目类别:
-
资助金额:$30.08万
-
财政年份:2005
-
负责人:Raju S. Kucherlapati
-
依托单位:
Comparative Genetics of the DiGeorge Syndrome Gene TBX1
-
批准号:6830948
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项目类别:
-
资助金额:$31.63万
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财政年份:2005
-
负责人:Raju S. Kucherlapati
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依托单位:
PTPN11: GENOTYPE PHENOTYPE CORRELATIONS IN NOONAN SYNDROME & RELATED DISORDERS
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批准号:7205075
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项目类别:
-
资助金额:$0.19万
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财政年份:2004
-
负责人:Raju S. Kucherlapati
-
依托单位:
海外基金