COMPREHENSIVE ASSESSMENT OF PTS WITH SUSPECTED CHRONIC LYME INFECTION

该研究正在组建一个特征良好的队列 推测患有慢性莱姆病和相关疾病的患者 控制。这些患者正在接受广泛的评估 横断面研究和神经疏螺旋体病患者将 静脉注射头孢曲松治疗后进行前瞻性随访。 这些研究将产生一个前瞻性数据库 可以建立严格的诊断标准，并在未来 可以设计治疗试验。目前，已有32名患者和 对照已被纳入该研究。这项研究还处于早期阶段 发展阶段，现在下结论还为时过早 多重结果分析得出的结论 对入组患者进行了广泛的测试。多项研究 正在根据本报告的调查结果制定项目 人口。所有这些项目都正在进行中。与合作 临床部的 Stephen Fischer 博士和 Susan Weir 博士 沃伦·格兰特·马格努森临床病理科 中心开发了一种新的伯氏疏螺旋体 PCR 检测方法， 目前正处于临床应用的早期阶段。 PCR 被用于 寻找冷冻脑活检中是否存在疏螺旋体 阿尔茨海默病患者和匹配的对照者。这是 与来自的 Juan Troncoso 博士合作进行 JHU 提供了脑活检。与博士合作。 来自 NINDS 的 Roland Martin 和 Henry McFarland 博士， 伯氏疏螺旋体特异性 T 细胞克隆已从外周血中分离出来。 慢性病患者的血液、关节液和/或脑脊液 神经疏螺旋体病和莱姆关节炎。一些细胞因子的概况 对这些克隆进行了研究。此外，还研究了一种克隆 使用组合肽库来精细定义 反应性肽。与 NINDS 的 Grafman 博士合作， 我们正在研究患者的神经心理学发现 患有慢性莱姆病并控制。合作中 与 NIDCD 的 Choo 博士和 Lawrence Shotland 博士一起，我们 研究慢性莱姆病患者的听力学结果 疾病。与 NCI 的 Duray 博士和 来自 NICHD 的 Leonid Margolis，我们正在开发一个新系统 疏螺旋体的体外培养。该系统可以使文化 与伯氏疏螺旋体最密切相关的环境 疏螺旋体在人类宿主中遇到的不同环境。 这些对于理解该病的发病机制具有重要意义。 体内感染。

The study is assembling a well-characterized cohort of patients with presumed chronic Lyme disease and relevant controls. These patients are being extensively evaluated in a cross-sectional study and the neuroborreliosis patients will be followed prospectively after therapy with intravenous ceftriaxone. These studies will yield a prospective database upon which stringent diagnostic criteria can be established and future therapeutic trials can be designed. At this point, 32 patients and controls have been enrolled in the study. This study is in its early phase of development, and, at this point, it is too early to draw conclusions from the analysis of the results of the multiple and extensive testing done in the enrolled patients. Multiple research projects are being developed based on the findings in this population. All of these projects are ongoing. In collaboration with Dr. Stephen Fischer and Dr. Susan Weir from the Clinical Pathology Department at the Warren Grant Magnuson Clinical Center, a new PCR assay for Borrelia has been developed and is now in early phase of clinical application. The PCR is being used to seek for the presence of Borrelia in frozen brain biopsies from patients with Alzheimer?s disease and matched controls. This is being performed in collaboration with Dr. Juan Troncoso from JHU, who provided the brain biopsies. In collaboration with Dr. Roland Martin and Dr. Henry McFarland from NINDS, Borrelia-specific T cell clones have been isolated from peripheral blood, joint and/or cerebrospinal fluid from patients with chronic neuroborreliosis and Lyme arthritis. The cytokine profiles of some of these clones have been studied. Also, one clone has been studied using combinatorial peptide libraries for fine definition of the reactive peptide. In collaboration with Dr. Grafman from NINDS, we are studying the neuropsychological findings of patients suffering from chronic Lyme disease and controls. In collaboration with Dr. Choo and Dr. Lawrence Shotland from NIDCD, we are studying the audiological findings in patients with chronic Lyme disease. In collaboration with Dr. Duray from NCI and with Dr. Leonid Margolis from NICHD, we are developing a new system for cultivation of Borrelia in vitro . This system may enable the culture of Borrelia in an environment that is most closely related to the different environments that Borrelia encounters in the human host. These will be important in the understanding of the pathogenesis of infection in vivo.