STRUCTURAL BASIS FOR STIMULUS CODING IN THE COCHLEAR NUCLEUS

耳蜗核刺激编码的结构基础

• 批准号: 6104384
• 负责人: DAVID K. RYUGO
• 金额: $ 10.15万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6104384
• 关键词: G protein; auditory nuclei; biological signal transduction; brain mapping; cats; cell type; electron microscopy; electrophysiology; evoked potentials; hearing; immunocytochemistry; intercellular connection; laboratory rat; membrane channels; neural information processing; neuroanatomy; neurochemistry; neurons; pons; single cell analysis; synapses; synaptic vesicles

Our long-term goals are to understand neuronal mechanisms that underlie hearing in mammals. In the auditory nervous system, all acoustic information from the environment enters the brain by passing from the auditory nerve and synapsing in the cochlear nucleus. In the cochlear nucleus, the relatively homogeneous responses of incoming auditory nerve fibers are transformed into a variety of different response patterns by the different classes of resident neurons, and the resultant signals are in turn transmitted to higher centers. The spectrum of these responses is hypothesized to depend upon the synaptic organization of auditory nerve input, intrinsic neurons, and descending inputs; the types and distribution of receptors, ion channels, and G proteins; and second messengers, all of which form the signaling capabilities in each cell class. In order to understand the how sound is processed at this key auditory nucleus, there is a need to study identified cell populations, to analyze their synaptic connections, and to reveal features of their signal processing capabilities. The present proposal will apply anterograde and retrograde tract tracing methods to identify neurons and their circuits, and immunocytochemical staining procedures to reveal the chemistry of these different neuron populations. In addition, we will apply in vivo and in vitro intracellular recording and staining methods in order to reveal structure-function relationships in the cochlear nucleus at the single cell level. The data from these projects will provide insights into how neurons and their circuits shape the coding process in the central auditory system, and could help in the design of a cochlear nucleus implant for deaf individuals who cannot benefit from a cochlear prothesis.

我们的长期目标是了解 哺乳动物的听力在听觉神经系统中， 来自环境的信息通过从大脑的 耳蜗核内的听神经和突触。耳蜗 神经核，传入听神经的相对均匀的反应 纤维被转换成各种不同的反应模式， 不同种类的常驻神经元，以及由此产生的信号是 反过来又传递到更高的中心。这些反应的范围是 假设依赖于听觉神经的突触组织 输入，内在神经元和下行输入;类型和 受体、离子通道和G蛋白的分布;以及第二 信使，所有这些都形成了每个细胞的信号能力， 课为了了解声音在这个调上是如何处理的 听觉核，有必要研究确定的细胞群， 分析它们的突触连接，并揭示它们的信号特征， 处理能力。 目前的建议将应用顺行和逆行追踪 识别神经元及其回路的方法，以及免疫细胞化学 染色程序来揭示这些不同神经元的化学性质 人口。此外，我们将在体内和体外细胞内应用 记录和染色方法，以揭示结构-功能 耳蜗核在单细胞水平上的关系。数据 这些项目将提供关于神经元及其 回路塑造了中央听觉系统中的编码过程， 可以帮助设计耳蜗核植入物， 无法从人工耳蜗中受益的人。