GLYCEROL METABOLISM IN 60-HR FASTED STATE

60 小时禁食状态下的甘油代谢

• 批准号: 6278644
• 负责人: M D JENSEN
• 金额: $ 2.03万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6278644
• 关键词: adipose tissue; carbohydrate metabolism; clinical research; esterification; fasting; fatty acid metabolism; free fatty acids; gluconeogenesis; glycerol; human subject; kidney metabolism; lipid biosynthesis; lipolysis; liver metabolism; triglycerides; very low density lipoprotein

The purpose of this study is to determine how the body uses glycerol during fasting. The specific aims are: 1) determine whether the uptake of FFA relative to glycerol in the splanchnic and renal beds during fasting are consistent with the need for systemic FFA re-esterification; 2) assess the contribution of splanchnic adipose tissue lipolysis to systemic FFA availability during fasting; and 3) examine whether glycerol used by the liver to synthesize VLDL triglyceride originates from a common intrahepatic gluconeogenic precursor pool or from circulating free glycerol. Four volunteers have been included in this study. Glycerol turnover and the incorporation of glycerol into the backbone of VLDL triglyceride was measured in these four volunteers. As part of the study the volunteers received deuterated water in order to examine the incorporation of deuterium into the glycerol and glucose in plasma. These samples were sent to Dr. Bernard Landau at Case Western Reserve. We have completed the glycerol analysis at Mayo.

这项研究的目的是确定身体如何利用甘油 禁食期间。 具体目标是：1）确定是否摄取 内脏和肾床中 FFA 相对于甘油的含量 禁食与全身 FFA 再酯化的需要一致； 2）评估内脏脂肪组织脂肪分解的贡献 禁食期间全身 FFA 的可用性； 3）检查是否 肝脏用来合成 VLDL 甘油三酯的甘油来源 来自共同的肝内糖异生前体库或来自 循环游离甘油。 这项研究包括四名志愿者。 甘油周转率和 将甘油掺入 VLDL 甘油三酯的骨架中 对这四名志愿者进行了测量。 作为研究的一部分，志愿者 接受氘化水以检查掺入 氘分解成血浆中的甘油和葡萄糖。 这些样本是 发送给凯斯西储的伯纳德·兰道博士。 我们已经完成了 梅奥的甘油分析。