TURNOVER AND OXIDATION OF LEG INTRACELLULAR FATTY ACID POOLS

腿部细胞内脂肪酸池的周转和氧化

• 批准号: 6117417
• 负责人: M D JENSEN
• 金额: $ 2.01万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117417
• 关键词: clinical research; fatty acid metabolism; fatty acid transport; human subject; leg; liver metabolism; muscle metabolism; oxidation

The purpose of this study is to determine how muscle takes up and burns fat present in the bloodstream. Participation is limited to persons who have maintained a constant body weight for two months prior to the study. The specific aims of this study are to determine whether: 1) Differences in resting energy expenditure, as reflected by differences in basal VO2 predict inter-individual differneces in average basal FFA flux in lean, healthy adults; 2) Gender and race (Caucasian vs African-American) influence the relationship between average VO2 and average basal FFA flux; 3) Obesty, upper body fat distribution and increased fat cell size are associated with increased fat cell size are associated with increased average basal FFA flux relative to VO2 in Caucasian and African-American adults; 4) Hepatic FFA delivery is increased in glucose intolerant (NIDDM) hypertriglyceridemic individuals (obese and non-obese) compared with glucose tolerant, nonmolipedemic individuals (obese and non-obese); 5) Intracellular muscle fatt acid fractional turnover rates and pools sizes predicted from leg arterio-venous difference techniques correlate with values obtained from muscle biopsy of the same leg; 6) Intracellular fatty acid fractional turnover rates and pools sizes in muscle are greater in obese, glucose intolerant and lean, glucose tolerant individuals. Since we began this protocol, we have developed new methods and techniques that have convinced us it is no longer justified to continue with this study. The information we would have gained from this study is being collected.

这项研究的目的是确定肌肉如何吸收和燃烧血液中存在的脂肪。参与者仅限于在研究前保持恒定体重两个月的人。本研究的具体目的是确定：1)基础VO2反映的静息能量消耗的差异是否预示着健康瘦削成年人基础FFA平均流量的个体间差异；2)性别和种族(高加索人与非裔美国人)影响平均VO2与平均基础FFA流量之间的关系；3)肥胖、上半身脂肪分布和脂肪细胞大小与脂肪细胞大小增加相关，高加索人和非裔美国成年人的平均基础FFA流量相对于VO2增加；4)糖耐量异常(NIDDM)高甘油三酯血症患者(肥胖和非肥胖)与糖耐量正常的非糖耐量人群(肥胖和非肥胖)相比，肝脏FFA的释放增加；5)由腿动静脉差值技术预测的细胞内肌肉脂肪酸周转率和池大小与同一条腿肌肉活检获得的值相关；6)肥胖、糖耐量异常和精瘦、糖耐量异常的个体细胞内脂肪酸的周转率和池大小更大。自从我们开始这个方案以来，我们开发了新的方法和技术，使我们相信不再有理由继续这项研究。我们将从这项研究中获得的信息正在收集中。