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STRUCTURE OF HYPERTROPHIC CENTROSOMES IN HUMAN BREAST TUMORS

人乳腺肿瘤肥大中心体的结构

基本信息

批准号：
6282142
负责人：
JEFFREY L SALISBURY
金额：
$ 2.95万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
起止时间：
1998-04-01 至 1999-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6282142
关键词：
biomedical resource clinical research female human subject microscopy neoplasm /cancer reproductive system women's health

项目摘要

We have shown through immunofluorescence and confocal microscopy that centrosomes of high grade breast tumors are larger and more numerous than centrosomes in normal breast tissue. Additionally, the centrosomes in breast tumor cells are hyperphosphorylated and are capable of nucleating a larger number of microtubules in vitro as compared to centrosomes from normal breast cells. We describe these centrosomes as "hypertrophic". Attempts to characterize hypertrophic centrosomes at an ultrastructural level using CTEM have been difficult. This is due, at least in part, to the small volume of the centrosomes relative to the total volume of the cells; the centrosomes are few and far between in 90 nm thick sections through cells that are 6-10 um thick. In addition, the hypertrophic centrosomes we have observed with CTEM have been especially electron opaque, making it difficult to resolve microtubules and other structural components of the centrosomes. Initial results using 0.5 and 1 micron thick sections have proven not only to greatly facilitate the locating of hypertropic centrosomes, but have also produced 3-dimensional images with heretofore unseen structural detail. Several tomographic reconstructions of centrosomes from cancerous cells were performed when Dr. Lingle visited the laboratory last summer. Additional reconstructions are planned in the near future.

我们通过免疫荧光和共聚焦显微镜高级别乳腺肿瘤的中心体更大，比正常乳腺组织中的中心体多。另夕h 乳腺肿瘤细胞中的中心体被过度磷酸化，能够在体外使更多的微管成核，与正常乳腺细胞的中心体相比。描述此等中心体“肥大”。试图描述肥大的特征使用CTEM在超微结构水平上观察中心体，难这是由于，至少部分是由于，相对于细胞总体积的中心体;中心体在90 nm厚的切片中， 6-10嗯厚。另外，我们发现的肥大中心体用CTEM观察到的是电子不透明的，很难分辨微管和其他结构成分中心体使用0.5和1微米厚的初始结果事实证明，不仅大大方便了肥大的中心体，但也产生了三维图像有着前所未有的结构细节几个断层扫描进行癌细胞中心体的重建林格尔博士去年夏天访问实验室时额外计划在不久的将来进行重建。