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HIGH FIELD EPR OF SPIN LABELED HUMAN GROWTH HORMONE

HIGH FIELD EPR OF SPIN LABELED HUMAN GROWTH HORMONE
自旋标记人类生长激素的高场 EPR
批准号：
6120625
负责人：
ALEX I. SMIRNOV
金额：
$1.62万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
1998
资助国家：
美国
项目状态：
已结题
起止时间：
1998-04-15 至 1999-11-30
来源：
https://reporter.nih.gov/project-details/6120625
关键词：
biological products biomedical resource hormones technology /technique

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英文摘要
The Utility of spin-labeling methods and high resolution 95 GHz (W-band) EPR is demonstrated as applied to equilibrium denaturing of spin-labeled recombinant human growth hormone. Experiments were carried out with 70 nanoliter vlume 33 M aqueous spin-labeled protein samples in a high-quality-factor (Qv4000) cylindrical resonator. Concentrational sensitivity was comparable to that of X-band (9.5 GHz) EPR. The amount of spin-labeled protein in W-band experiments was only v2 picoMoles. At W-band the field separation observed for spin label spectra corresponding to native and denatured protein states was 0.6G which, in principle, allows resolution of the states with interconversion rate as fast as v5 sec. Denaturing curves were derived from isotropic g-factor and line width data and analyzed under a two state model.
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Upgrade of Bruker Time-domain EPR Spectrometer
Nanodiamond Quantum Sensors for Free Radical Detection
  • 批准号：
    10325762
  • 项目类别：
  • 资助金额：
    $25.66万
  • 财政年份：
    2021
  • 负责人：
    ALEX I. SMIRNOV
  • 依托单位：
    ADAMAS NANOTECHNOLOGIES, INC.
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PROTEIN DEPOSITION ONTO LIPID NANOTUBE ARRAYS
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