W BAND EPR SPECTROMETER DEVELOPMENT

W 波段 EPR 光谱仪的开发

• 批准号: 6120597
• 负责人: MARK R NILGES
• 金额: $ 2.7万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120597
• 关键词: biomedical equipment development; biomedical resource; technology /technique

Multi-purpose variable temperature setup (2.5-300K) for wide-scan (up 7 T) W-band EPR spectroscopy has been engineered, tested, and is in operation. It is frequently improved and upgraded. Setup utilizes a vertically movable remotely controlled platform with a 95 GHz microwave bridge, a modified continuous flow Oxford cryostat CF 1200 outfitted with custom-implemented microwave, electrical and mechanical feedthroughs, modified I TC-4 Oxford temperature controller, and a multi-purpose W-band probehead with a remote tuning and a goniometer for one-axis sample rotation. The setup is suitable for High Frequency EPR spectroscopy of metalloproteins at cryogenic temperatures, including studies of single crystals of metalloproteins with submillimeter sizes. The setup is available for outside users. During the 1996 year we have designed and manufactured a new resonator for low-temperature measurements which does not show any detectable impurities signal compared with previous design. This year, progress includes 1) development of the next version of the probehead with built-in goniometer allowing 0.1 degree accuracy in sample positioning. 2) Implementing dielectric waveguide section to cut microwave and thermal losses during cryogenic EPR experiments. 3) Development of the probehead for sub-helium work.

多用途可变温度设置（2.5- 300 K），适用于宽扫描 (up 7 T）W波段EPR光谱已被设计，测试， 运行中。 它经常被改进和升级。 设置利用 一个垂直移动的遥控平台， 微波桥，改进的连续流牛津低温恒温器CF 1200 配备了定制的微波，电气和机械 反馈器，改进的I TC-4牛津温度控制器，以及 带远程调谐和测角仪的多用途W波段探头 用于单轴样品旋转。 该设置适用于高 低温下金属蛋白质的频率EPR谱 温度，包括金属蛋白单晶的研究 亚毫米大小的 该设置可供外部用户使用。 在1996年期间，我们设计并制造了一种新的谐振器 对于没有显示任何可检测的 与以前的设计相比，杂质信号。 今年以来， 进展包括：1）开发下一个版本的探头 内置角度计，样品精度为0.1度 定位 2)实现介质波导段切割 低温EPR实验中的微波和热损耗。 第三章 亚氦工作探头的研制。