EARLY BONE FORMATION AT BONE BIOMATERIAL INTERFACE

骨生物材料界面的早期骨形成

• 批准号: 6259343
• 负责人: JOO L. ONG
• 金额: $ 30.09万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6259343
• 关键词: X ray crystallography; biomaterial interface interaction; biomaterials; calcium ion; cell differentiation; cell proliferation; dental implants; fibronectins; flow cytometry; hydroxyapatites; infrared spectrometry; interferometry; laboratory rabbit; laboratory rat; medical implant science; nucleic acid probes; oligonucleotides; osteoblasts; osteogenesis; osteopontin; phosphates; polymerase chain reaction; titanium

DESCRIPTION (Verbatim from the Applicant): The goal of this research is to gain a better understanding of the biological basis for successful orthopaedic and dental implant therapy by elucidating the early phenomena that govern osseointegration. In this proposal, the effect of sputtered hydroxyapatite (HA) crystallinity on early bone cell activity in vitro and in vivo will be investigated under highly controlled and defined conditions. Our overall hypothesis is that, under conditions where other variables are controlled, the degree of crystallinity of the HA surface directly affects early bone cell activity in vitro and the rate of development of osseointegration in vivo. The objective of this study is to correlate the effect of characterized HA crystallinity to dissolution and protein adsorption, bone cell response in vitro and early cell activities in vivo. In this proposal, Aim 1 will be to determine the relationship between crystalline content of well-characterized HA surfaces and 1) the adsorption of specific extracellular matrix proteins, fibronectin and osteopontin, and 2) the rate of dissolution of the surface. The HA and Ti coatings will be produced using sputter coating. The rationale for using the sputtering technology is due to the high coating-metal adhesion strength compared to plasma spraying. Protein adsorption and dissolution of the coatings will be measured over time. Aim 2 will determine the extent to which the crystalline content of HA surfaces effects osteoblast proliferation, differentiation, and metabolism in vitro. It is hypothesized in this aim that because osteoblast proliferation and differentiation may be affected by either the adsorption of specific extracellular matrix proteins, fibronectin and osteopontin, or the rate of dissolution of the surface, or both; metabolic activity leading to mineral formation will vary with the crystalline content of the HA surface. Implicit in this hypothesis is there exists an optimal crystalline content of an HA surface for the promotion of bone formation activity. Aim 3 will evaluate the extent to which the crystalline content of HA surfaces affects osseointegration in vivo. Early bone activity will be evaluated using histology, mechanical strength and immunohistochemistry in this aim. Data generated from this study will provide information on the early maturation of bone cells in the presence of implant biomaterials and will provide a correlation between biomaterial properties and bone cell responses in vitro and in vivo. Additionally, information generated will contribute to the development of an ideal implant surface, thereby reducing long-term implant failures.

描述(来自申请者的逐字)：这项研究的目标是获得 更好地了解成功的骨科和外科手术的生物学基础 通过阐明控制早期现象的牙种植体治疗 骨整合。在这个方案中，溅射的羟基磷灰石(HA)的效果 结晶度对早期骨细胞体外和体内活性的影响 在严格控制和限定的条件下进行调查。我们的整体 假设是，在控制其他变量的条件下， 羟基磷灰石表面的结晶度直接影响早期骨细胞 体外活性和体内骨结合发育速度。这个 本研究的目的是关联特征性HA的效果 对溶解和蛋白质吸附的结晶度，骨细胞的反应 体外和体内早期细胞活性。在这项提案中，目标1将是 确定表征良好的透明质酸结晶含量之间的关系 表面和1)特定细胞外基质蛋白的吸附， 纤维连接蛋白和骨桥蛋白，以及2)表面的溶解速度。这个 采用溅射镀膜的方法制备HA和Ti涂层。其基本原理是 采用溅射技术是因为涂层与金属的附着力很高 与等离子喷涂相比，其强度更高。蛋白质的吸附和溶解 涂层将随着时间的推移进行测量。目标2将决定在何种程度上 HA表面的结晶含量影响成骨细胞的增殖， 分化，并在体外代谢。在这个目标中假设 因为成骨细胞的增殖和分化可能受到任何一种因素的影响 特异性细胞外基质蛋白、纤维连接蛋白和 骨桥蛋白，或表面的溶解速度，或两者兼而有之；代谢 导致矿物形成的活动将随晶体含量的不同而变化 HA表面。隐含在这个假设中的是存在一个最优的 羟基磷灰石表面促进骨形成的结晶含量 活动。目标3将评估HA结晶含量在多大程度上 表面影响体内的骨整合。早期骨骼活动将是 用组织学、机械强度和免疫组织化学方法评价 瞄准。这项研究产生的数据将提供关于早期 植入生物材料和Will存在下骨细胞的成熟 提供生物材料特性和骨细胞响应之间的相关性 体外和体内。此外，生成的信息将有助于 开发理想的种植体表面，从而减少长期种植体 失败。