EARLY BONE FORMATION AT BONE BIOMATERIAL INTERFACE

骨生物材料界面的早期骨形成

• 批准号: 6863766
• 负责人: JOO L. ONG
• 金额: $ 14.75万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2006-08-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6863766
• 关键词: X ray crystallography; biomaterial interface interaction; biomaterials; calcium ion; cell differentiation; cell proliferation; dental implants; fibronectins; flow cytometry; hydroxyapatites; infrared spectrometry; interferometry; laboratory rabbit; laboratory rat; medical implant science; nucleic acid probes; oligonucleotides; osteoblasts; osteogenesis; osteopontin; phosphates; polymerase chain reaction; titanium

DESCRIPTION (Verbatim from the Applicant): The goal of this research is to gain a better understanding of the biological basis for successful orthopaedic and dental implant therapy by elucidating the early phenomena that govern osseointegration. In this proposal, the effect of sputtered hydroxyapatite (HA) crystallinity on early bone cell activity in vitro and in vivo will be investigated under highly controlled and defined conditions. Our overall hypothesis is that, under conditions where other variables are controlled, the degree of crystallinity of the HA surface directly affects early bone cell activity in vitro and the rate of development of osseointegration in vivo. The objective of this study is to correlate the effect of characterized HA crystallinity to dissolution and protein adsorption, bone cell response in vitro and early cell activities in vivo. In this proposal, Aim 1 will be to determine the relationship between crystalline content of well-characterized HA surfaces and 1) the adsorption of specific extracellular matrix proteins, fibronectin and osteopontin, and 2) the rate of dissolution of the surface. The HA and Ti coatings will be produced using sputter coating. The rationale for using the sputtering technology is due to the high coating-metal adhesion strength compared to plasma spraying. Protein adsorption and dissolution of the coatings will be measured over time. Aim 2 will determine the extent to which the crystalline content of HA surfaces effects osteoblast proliferation, differentiation, and metabolism in vitro. It is hypothesized in this aim that because osteoblast proliferation and differentiation may be affected by either the adsorption of specific extracellular matrix proteins, fibronectin and osteopontin, or the rate of dissolution of the surface, or both; metabolic activity leading to mineral formation will vary with the crystalline content of the HA surface. Implicit in this hypothesis is there exists an optimal crystalline content of an HA surface for the promotion of bone formation activity. Aim 3 will evaluate the extent to which the crystalline content of HA surfaces affects osseointegration in vivo. Early bone activity will be evaluated using histology, mechanical strength and immunohistochemistry in this aim. Data generated from this study will provide information on the early maturation of bone cells in the presence of implant biomaterials and will provide a correlation between biomaterial properties and bone cell responses in vitro and in vivo. Additionally, information generated will contribute to the development of an ideal implant surface, thereby reducing long-term implant failures.

描述（申请人的逐字记录）：本研究的目标是获得 更好地理解成功骨科手术的生物学基础， 牙科种植体治疗通过阐明早期现象， 骨整合在这个建议中，溅射羟基磷灰石（HA）的效果， 结晶度对体外和体内早期骨细胞活性的影响 在严格控制和规定的条件下进行调查。我们的整体 假设是，在控制其他变量的条件下， HA表面的结晶度直接影响早期骨细胞 体外活性和体内骨整合的发展速率。的 本研究的目的是将表征的HA的作用 结晶度溶解和蛋白质吸附，骨细胞反应， 体外和体内早期细胞活性。在本提案中，目标1将是 确定良好表征的HA的结晶含量之间的关系 表面和1）特异性细胞外基质蛋白的吸附， 纤连蛋白和骨桥蛋白，和2）表面溶解速率。的 HA和Ti涂层将使用溅射涂层生产。的理由 使用溅射技术是由于高的涂层-金属附着力 与等离子喷涂相比。蛋白质的吸附和溶解 将随时间测量涂层。目标2将决定 HA表面的结晶含量影响成骨细胞增殖， 分化和体外代谢。在这一目标中假设， 因为成骨细胞的增殖和分化可能受到 特异性细胞外基质蛋白、纤连蛋白和 骨桥蛋白，或表面溶解速率，或两者;代谢 导致矿物形成的活性将随着晶体含量的变化而变化。 HA表面。这个假设隐含的意思是存在一个最佳的 用于促进骨形成的HA表面的结晶含量 活动目的3将评估HA的结晶含量 表面影响体内骨整合。早期骨活动将是 使用组织学、机械强度和免疫组织化学进行评价， 瞄准本研究产生的数据将提供有关早期 骨细胞在植入生物材料存在下的成熟， 提供了生物材料特性和骨细胞反应之间的相关性， 体外和体内。此外，所产生的信息将有助于 形成理想的种植体表面，从而减少长期种植 失败