LARGE-SCALE PRODUCTION & PURIFICATION OF COMPOUNDS WITH BIOLOGICAL ACTIVITY

生物技术股负责生物材料的生产和提纯。该股承担了广泛的生物生产任务，包括大规模培养原核细胞和真核细胞，从微生物和真核细胞中生产各种化合物，以及提纯生物化合物，特别是蛋白质。这些产品无法从商业渠道获得，但需要用于临床和结构研究。生物技术股利用生理和技术方法处理与重组产品和本地产品的生长和表达有关的各种生产问题。由于该股拥有两个综合要素：全面运作的多用途试生产设施和进行研究和工艺开发的能力，因此有可能进行有效的工艺开发和生产。在过去的一年里，该部门进行了大约200种不同的大规模准备工作，包括：1、培养各种微生物，如重组大肠杆菌、重组酿酒酵母、重组毕赤酵母、百日咳杆菌突变株、白喉棒状杆菌突变株以及几种类型的沙门氏菌和志贺氏菌。2，大量培养各种哺乳动物细胞；3，纯化克量的白喉毒素CRM(交叉反应材料)和重组铜绿假单胞菌外毒素A；4，利用填充床生物反应器，从重组293细胞中生产人甲状旁腺钙受体的胞外区。在研发领域，该单位继续：1，致力于开发更有效的大规模蛋白质回收方法，实施扩展床吸附技术的实验，以在离子交换器和螯合树脂上捕获蛋白质；2，利用核磁共振方法，进行旨在了解各种重组大肠杆菌菌株的葡萄糖代谢的研究，以找出为什么某些菌株更适合于外国蛋白质生产。3、建立果蝇细胞生产重组蛋白的发酵程序；4、了解和改进毕赤酵母重组蛋白的表达过程，特别是内皮抑素和血管抑素的表达过程。-发酵、纯化、细胞培养、生化工程

The Biotechnology Unit is responsible for the production and purification of biological material. The Unit undertakes a wide array of biological production tasks including large-scale growth of prokaryotic cells and eucaryotic cells, production of various compounds from microorganisms and eucaryotic dells, and purification of biological compounds especially proteins. These products, not available from commercial sources, are needed for clinical and structural studies. The Biotechnology Unit handles various production problems associated with growth and expression of recombinant and native products using both physiological and technical approaches. Efficient process development and production is possible since the Unit possesses two integrated elements: a fully operational multi-purpose pilot production facility and the capability of conducting research and process development. During the last year the unit performed approximately 200 different, large scale preparations including: 1, growing various microorganisms such as recombinant Escherichia coli, recombinant Saccharomyces cerevisiae, recombinant Pichia pastoris, mutant strains of Bortedella pertussis, mutant strains of Corynebacteria diphtheria and several types of Salmonella and Shigella. 2, propagating large volumes of various mammalian cells; 3, purifying gram quantities of diphtheria toxin CRM (Cross Reacting Material) and recombinant Pseudomonas aeruginosa exotoxin A, and 4, producing the extracellular domain of the human parathyroid Ca++ receptor from recombinant 293 cells using packed bed bioreactor. In the research and development arena the unit continues: 1, its effort on the development of more efficient methods for large scale protein recovery, performing experiments implementing expanded bed adsorption techniques for capturing proteins on an ion exchangers and chelating resins; 2, conducting research aimed at understanding the glucose metabolism of various recombinant E. coli strains in an effort to find out why certain strains are more suitable for foreign protein production, using NMR methods. 3, developing fermentaion procedures of Drosophila cells for the production of recombinant proteins and 4, understanding and improving of the process of recombinant protein expression from Pichia pastoris especially endostatin and angiostatin. - Fermentation, Purification, cell culture, biochemical engineering