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Large-scale production and purification of biological compounds

生物化合物的大规模生产和纯化

基本信息

批准号：
9148972
负责人：
Joseph Shiloach
金额：
$ 59.58万
依托单位：
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9148972
关键词：
Bacillus (bacterium)Bacteria Biocompatible Materials Biological Bioreactors Biotechnology Cell Density Cells Cells Plant Tissue Clinical Development Escherichia coli Fermentation Goals Growth Hela Cells Human Insecta Laboratory Research Maintenance Mammalian Cell Membrane Proteins Methodology Methods National Institute of Diabetes and Digestive and Kidney Diseases Operative Surgical Procedures P-Glycoprotein Peptidoglycan Pichia Procedures Process Production Recombinant Proteins Recombinants Recovery Research Research Project Grants Saccharomyces cerevisiae Source T cell therapy Testing Tissues Transfection United States National Institutes of Health Universities Yeasts base drug development improved instrumentation large scale production medical schools microorganism operation research study structural biology vaccine development vector

项目摘要

Summary: An important portion of the Lab activities is the production of high-quality and unique biologicals that are not available from commercial sources. On average, the Unit performs around 100 processes last year, which include fermentation of bacteria, yeast, insect cells, and mammalian cells (in volumes ranging from 2 to 100 liters), and initial recovery and purification of biomolecules. The following are examples of processes performed: growth of bacteria such as Escherichia coli, (recombinant and native) various recombinant yeast strains such as Saccharomyces cerevisiae and Pichia pastoris. In addition, mammalian cells such as HeLa, CHO, MDCK and HEK 293, insect cell such Sf 9, and High five for transient expression of recombinant proteins were propagated. The various products were needed for a number of collaborative research projects: Expression of P-glycoprotein (membrane protein transporter) from pichia pastoris (NCI), expression of human NPP1 in HEK 293 cells (Yale university medical school), and peptidoglycan from Bacillus anthraces (NIH clinical center). The lab initiated a new project together with the surgery branch of the NCI for improvement of the method currently used for production of gammaretroviral vectors needed for adoptive T cell therapy. The existing procedure is based on production from adherent packaging cells growing on stationary support which is a rate limiting step in this research. Our aim is to improve the growth procedure by testing various methodologies using stirred tank reactor. Initial experiments were successfully conducted for the growth of the packaging cells in bioreactors

简介: