Large-scale production and purification of biological compounds

实验室活动的很大一部分是生产无法从商业来源获得的高质量和独特的生物制剂。该单位平均每年进行约100道工序，其中包括细菌、酵母、昆虫细胞和哺乳动物细胞的发酵（体积从2升到200升不等），以及从不同来源回收和纯化生物分子，如蛋白质和多糖。以下是所进行过程的示例：细菌的生长，如大肠杆菌，（重组和原生），沙门氏菌，志贺氏菌，葡萄球菌，流感嗜血杆菌，脑膜炎奈瑟菌（脑膜炎球菌），伯氏疏螺旋体，盐杆菌，支杆菌sp，微杆菌sp，以及各种重组酵母菌菌株，如毕赤酵母。此外，还对HeLa、CHO、MDCK、HEK 293等哺乳动物细胞和sf9、High 5等昆虫细胞进行了瞬时表达重组蛋白的繁殖。以克为单位生产和纯化了几种蛋白质。例如：用于疟疾疫苗研究的重组艰难梭菌毒素A、重组人脂联素、重组铜绿假单胞菌外毒素A、重组白喉毒素和重组疟原虫蛋白。

A significant portion of the Labs activities is the production of high-quality and unique biologicals that are not available from commercial sources. On average, the Unit performs around 100 processes per year, which include fermentation of bacteria, yeast, insect cells, and mammalian cells (in volumes ranging from 2 to 200 liters), and recovery and purification of biomolecules, such as proteins and polysaccharides, from different sources. The following are examples of processes performed: growth of bacteria like Escherichia coli, (recombinant and native), Salmonella, Shighella, Staphylococcus, Haemophilus influenzae, N. meningitidis (meningococcus), Borrelia burgdorferi, Halobacteria, Bronchaseptica sp, Micobacteria sp, and various recombinant yeast strains like Pichia pastoris. In addition, mammalian cells such as HeLa, CHO, MDCK and HEK 293, and insect cell such Sf 9, and High five for transient expression of recombinant proteins were propagated. Several proteins were produced and purified in gram quantities. Examples are: recombinant Clostridium difficle toxin A, recombinant human adiponectin, recombinant pseudomonas aeruginasa exotoxin A, recombinant Diphtheria toxin and recombinant Plasmodium proteins for malaria vaccine research. The various products were needed for a number of collaborative research projects: human adiponectin was made for biological studies, the bacterial toxins for vaccine development and several proteins were produced for structural studies among them Toll proteins.