Large-scale production and purification of biological compounds

实验室活动的一个重要部分是生产商业来源无法获得的高质量和独特的生物制剂。平均而言，该单位每年执行约100个过程，其中包括细菌，酵母，昆虫细胞和哺乳动物细胞的发酵（体积从2到200升不等），以及从不同来源回收和纯化生物分子，如蛋白质和多糖。细菌如大肠杆菌（重组和天然的）、沙门氏菌属、志贺氏菌属、葡萄球菌属、流感嗜血杆菌、N.脑膜炎球菌（脑膜炎球菌）、伯氏疏螺旋体（Borrelia burgdorferi）、嗜盐菌（Halobacteria）、支气管炎菌属（Bronchaseptica sp）、微菌属（Micobacteria sp）和各种重组酵母菌株如巴斯德毕赤酵母（Pichia pastoris）。此外，繁殖哺乳动物细胞如HeLa、CHO、MDCK和HEK 293，以及昆虫细胞如Sf 9和High five，用于瞬时表达重组蛋白。几种蛋白质的生产和纯化克量。例如：用于疟疾疫苗研究的重组艰难梭菌毒素A、重组人脂联素、重组铜绿假单胞菌外毒素A、重组白喉毒素和重组疟原虫蛋白。 一些合作研究项目需要各种产品：人类脂联素用于生物学研究，细菌毒素用于疫苗开发，以及几种蛋白质用于结构研究，其中包括Toll蛋白。

A significant portion of the Labs activities is the production of high-quality and unique biologicals that are not available from commercial sources. On average, the Unit performs around 100 processes per year, which include fermentation of bacteria, yeast, insect cells, and mammalian cells (in volumes ranging from 2 to 200 liters), and recovery and purification of biomolecules, such as proteins and polysaccharides, from different sources. The following are examples of processes performed: growth of bacteria like Escherichia coli, (recombinant and native), Salmonella, Shighella, Staphylococcus, Haemophilus influenzae, N. meningitidis (meningococcus), Borrelia burgdorferi, Halobacteria, Bronchaseptica sp, Micobacteria sp, and various recombinant yeast strains like Pichia pastoris. In addition, mammalian cells such as HeLa, CHO, MDCK and HEK 293, and insect cell such Sf 9, and High five for transient expression of recombinant proteins were propagated. Several proteins were produced and purified in gram quantities. Examples are: recombinant Clostridium difficle toxin A, recombinant human adiponectin, recombinant pseudomonas aeruginasa exotoxin A, recombinant Diphtheria toxin and recombinant Plasmodium proteins for malaria vaccine research. The various products were needed for a number of collaborative research projects: human adiponectin was made for biological studies, the bacterial toxins for vaccine development and several proteins were produced for structural studies among them Toll proteins.