Large-scale production and purification of biological compounds

生物化合物的大规模生产和纯化

• 批准号: 7593413
• 负责人: Joseph Shiloach
• 金额: $ 30.26万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7593413
• 关键词: Adsorption; Bacillus anthracis; Bacteria; Beds; Biochemistry; Biological; Biotechnology; Cell Density; Cells; Cells Plant Tissue; Commercial Sources; Corynebacterium diphtheriae; Development; Engineering; Escherichia coli; Fermentation; G-Protein-Coupled Receptors; G-substrate; Genus staphylococcus; Growth; Hela Cells; Human; Immunotoxins; Influenza; Insecta; Laboratory Research; Maintenance; Mammalian Cell; Methods; Microbiology; Molecular Biology; National Institute of Diabetes and Digestive and Kidney Diseases; Numbers; Operative Surgical Procedures; Pichia; Polysaccharides; Preparation; Process; Production; Proteins; Pseudomonas aeruginosa toxA protein; Range; Recombinant Proteins; Recombinants; Recovery; Research; Research Project Grants; Saccharomyces cerevisiae; Salmonella; Source; T-Cell Depletion; Tissues; Vaccines; Yeasts; adiponectin; cancer type; drug development; instrumentation; microorganism; structural biology

A significant portion of the Units activities is the production of high-quality and unique biologicals that are not available from commercial sources. On average, the Unit performs between 150 to 200 processes per year, which include fermentation of bacteria, yeast, insect cells, and mammalian cells (in volumes ranging from 2 to 200 liters), and recovery and purification of biomolecules, such as proteins and polysaccharides, from different sources. The following are examples of processes performed: growth of bacteria like Escherichia coli, (recombinant and native), Corynebacteria diphtheria, Salmonella, Shighella, Staphylococcus, Haefmofillus influenzae, Bacillus Anthracis, and various recombinant yeast strains like Saccharomyces cerevisiae and Pichia pastoris. In addition, mammalian cells such as HeLa, CHO, BHK and HEK 293, and insect cells like Sf 9, and High five for transient expression of recombinant proteins were propagated. Several proteins were produced and purified in gram quantities. Examples are: recombinant Pseudomonas aeruginosa exotoxin A, and human adiponectin. The various products were needed for a number of collaborative research projects: the preparation of immunotoxins for the treatment of specific types of cancer and T cell depletion, human adiponectin was made for biological studies and several proteins were produced for structural studies among them Toll proteins and G protein-coupled receptors.

该单位的一项重要活动是生产商业来源无法获得的高质量和独特的生物制剂。平均而言，该单位每年进行150至200个过程，其中包括细菌，酵母，昆虫细胞和哺乳动物细胞的发酵（体积从2到200升不等），以及从不同来源回收和纯化生物分子，如蛋白质和多糖。以下是进行的过程的实例：细菌如大肠杆菌（重组和天然）、白喉棒状杆菌、沙门氏菌、斯海氏菌、葡萄球菌、流感嗜血杆菌、炭疽芽孢杆菌和各种重组酵母菌株如酿酒酵母和巴斯德毕赤酵母的生长。此外，繁殖哺乳动物细胞如HeLa、CHO、BHK和HEK 293，以及昆虫细胞如Sf 9和High five，用于瞬时表达重组蛋白。几种蛋白质的生产和纯化克量。例如：重组铜绿假单胞菌外毒素A和人脂联素。 许多合作研究项目都需要各种产品：制备用于治疗特定类型癌症和T细胞消耗的免疫毒素，为生物学研究制备人脂联素，并为结构研究生产了几种蛋白质，其中包括Toll蛋白和G蛋白偶联受体。