Large-scale production and purification of biological compounds

该股活动的很大一部分是生产无法从商业来源获得的高质量和独特的生物制品。平均而言，该单位每年进行150至200个工序，其中包括细菌、酵母、昆虫细胞和哺乳动物细胞的发酵（体积从2至200升不等），以及从不同来源回收和纯化生物分子，如蛋白质和多糖。以下是所进行过程的例子：细菌的生长，如大肠杆菌，（重组和原生），白喉棒状杆菌，沙门氏菌，志贺氏菌，葡萄球菌，流感嗜血杆菌，炭疽杆菌，以及各种重组酵母菌，如酿酒酵母菌和毕赤酵母。此外，还对HeLa、CHO、BHK、HEK 293等哺乳动物细胞和sf9、High five等昆虫细胞进行了瞬时表达重组蛋白的繁殖。以克为单位生产和纯化了几种蛋白质。例如：重组铜绿假单胞菌外毒素A和人脂联素。

A significant portion of the Units activities is the production of high-quality and unique biologicals that are not available from commercial sources. On average, the Unit performs between 150 to 200 processes per year, which include fermentation of bacteria, yeast, insect cells, and mammalian cells (in volumes ranging from 2 to 200 liters), and recovery and purification of biomolecules, such as proteins and polysaccharides, from different sources. The following are examples of processes performed: growth of bacteria like Escherichia coli, (recombinant and native), Corynebacteria diphtheria, Salmonella, Shighella, Staphylococcus, Haefmofillus influenzae, Bacillus Anthracis, and various recombinant yeast strains like Saccharomyces cerevisiae and Pichia pastoris. In addition, mammalian cells such as HeLa, CHO, BHK and HEK 293, and insect cells like Sf 9, and High five for transient expression of recombinant proteins were propagated. Several proteins were produced and purified in gram quantities. Examples are: recombinant Pseudomonas aeruginosa exotoxin A, and human adiponectin. The various products were needed for a number of collaborative research projects: the preparation of immunotoxins for the treatment of specific types of cancer and T cell depletion, human adiponectin was made for biological studies and several proteins were produced for structural studies among them Toll proteins and G protein-coupled receptors.